皂角刺中黄酮类化学成分的分离鉴定  

Isolation and structural elucidation of the flavonoids from the stings of Gleditsia sinensis L.

作　　者：李万华[1] 李琴 王小刚[3] 赵文明[1] 

机构地区：[1]西安交通大学生命科学与技术学院,陕西西安710049 [2]陕西省新药审评中心,陕西西安710054 [3]西北大学化工学院,陕西西安710069

出　　处：《西北大学学报（自然科学版）》2005年第6期763-765,770,共4页Journal of Northwest University（Natural Science Edition）

基　　金：陕西省科技攻关基金资助项目(2003K11-J9)

摘　　要：目的研究中药材皂角刺中黄酮类化合物化学成分。方法用溶剂萃取、反复柱层析方法,分离纯化皂角刺中黄酮类化合物,通过NMR,MS等波谱技术确定其化学结构,并进行细胞模型的体外活性筛选。结果从皂角刺中分离得到两种黄酮类化合物,结构鉴定为(+)trans-2R,3R-3′,4′,5,7-tetrahydroxyflavanonol(1)和8-C-glucopyranosyl-3,4′,7-trihydroxyflavone(2),化合物2显示出明显的组织蛋白酶B抑制活性(CAT-B模型)。结论该两种化合物均为首次从皂角刺中分离得到的单体黄酮类化合物,且发现化合物2具有组织蛋白酶B抑制活性。Aim To investigate the constituents of flavonoids from the stings of Gleditsia sinensis L. Methods The flavonoids were isolated by methods of extraction and repeated column chromatography, and their structure was elucidated on the basis of NMR, MS techniques. Their activities were examined by in-vitro bioassay. Results Two flavonoids were isolated from the stings of G. sinensis Lam. , their structure was elucidated as （ ＋ ）trans-2R ,3R- 3＇,4＇, 5,7-tetrahydroxyflavanonol （ 1 ） and 8-C-glucopyranosyl-3,4＇, 7-trihydroxy flavone （2）. Compound 2 showed moderate activities for inhibitting cathepsin B in the test on Cathepsin B bioassay （CAT-B）. Conclusion The two compounds were isolated from this herb for the first time, and the activities of compound 2 for inhibitting cathepsin B was found for the first time.

关 键 词：皂角刺 (+)trans-2R 3R-3’ 4’ 5 7-tetrahydroxyflavanonol 8-C—glucopyranosyl-3 4’ 7-trihydroxyflavone 

分 类 号：TQ460.72[医药卫生—药物分析学]