白血病患者外周血IL－2、mIL－2R和TNF－α的检测及意义  

DETECTION OF INTERLEUKIN-2,MEMBRANCE RECEPTORS FOR INTERLEUKIN-2,TUMOR NECROSIS FACTOR ALFA IN PERIPHERAL BLOOD AND THEIR SIGNIFICANCE IN LEUKEMIA PATIENTS

作　　者：李大启[1] 张爱华[1] 王宝珍[1] 高奇蓉[1] 胡美玉[1] 吴直江[1] 孙晓光[1] 陈可靖[1] 

机构地区：[1]山东省潍坊医学院附属医院,上海医科大学附属中山医院,中国科学院上海细胞生物所

出　　处：《白血病》1996年第3期157-159,共3页

摘　　要：４５例白血病患者，采用固相放射免疫分析法（ＲＩＡ）测定其血清白细胞介素２（ＩＬ－２）的水平，以流式细胞仪（ＦＣＭ）检测其外周血单个核细胞（ＰＭＮＣ）上膜白细胞介素２受体（ｍＩＬ－２Ｒ）的表达，用双抗体夹心ＣＥＬＩＳＡ法测定其血清肿瘤坏死固子α（ＴＮＦ－α）的含量。发现急性白血病（ＡＬ）及慢性位细胞白血病原始细胞危象（ＣＭＬ－ＢＣ）患者血清ＩＬ－２和ＴＮＦ－α的水平明显高于正常，ＣＭＬ－ＢＣ尤其急淋变者的ＰＭＮＣ上ｍＩＬ－２Ｒ阳性率较高，并与血清ＴＮＦ－α有关，而与血清ＩＬ－２无关，ｍＩＬ－２Ｒ阳性常提示白血病患者疗效差，预后不良。Serum interleukin-2(IL-2)and tumor necrosis factor-alfa(TNF-α)levels weremeasured in 45 leukemia pationts by using RIA and ELISA methods respectively;Exprcssion ofmembrane receptors for interleukin-2(mIL-2R)on peripheral blood mononuclear cells(PMVC)was also dertermined in patients using flowcytometry(FCM).Serum IL-2 and TNF-α were sig-nificantly increased in acute myeloid leukemia,acute lymphcytic leukemia and blast crisis ofchronic myelocytic leukcmia(CML-BC)paticnts,Positive mIL-2R exprcssion was higher inCML-BC than in others.Positive mIL-2R cxpression often indicatcd a bad clinical response orpoor prognosis in acute leukemia and CML-BC.A positive correlation between mIL-2R andTNF-α was also detected in CML-BC.

关 键 词：白血病 白细胞介素2 肿瘤坏死因子Α MIL-2R 

分 类 号：R733.7[医药卫生—肿瘤]