川芎嗪对哮喘大鼠NF-κB表达及气道炎症的影响  

Effect of ligustrazine on the expression of NF-κB and airway inflammation in asthmatic rats

作　　者：陈径[1] 杨莉[1] 金蕊[1] 王文建[1] 

机构地区：[1]东南大学附属中大医院儿科,江苏南京210009

出　　处：《现代医学》2005年第6期351-353,共3页Modern Medical Journal

摘　　要：目的研究川芎嗪对哮喘大鼠肺组织中核因子-κB(NF-κB)表达的影响及其抗炎作用。方法将SD大鼠随机分为4组:对照组、模型组、激素组和川芎嗪组。用卵蛋白复制大鼠哮喘模型;免疫组织化学技术观察气道上皮细胞NF-κB的表达;行支气管肺泡灌洗液(BALF)中嗜酸性粒细胞(Eos)计数及细胞分类计数。结果(1)4组NF-κB细胞核阳性表达的细胞占气道上皮细胞的百分比分别为(12.1±2.4)%、(26.8±3.6)%、(14.6±1.9)%和(17.3±2.6)%。模型组明显高于对照组(P<0.001);川芎嗪组明显低于模型组(P<0.01),且与激素组无显著性差异(P>0.05)。(2)川芎嗪组BALF中Eos的数目及Eos与淋巴细胞的比例均低于模型组(P<0.001),且其作用与激素组无显著性差异(P>0.05)。结论川芎嗪可以有效阻断肺组织中NF-κB的激活,减轻气道炎症。Objective To explore the effects of ligustrazine on nuclear factor-kappe B （NF-κB） expression and airway inflammation in a rat model of bronchial asthma. Methods Forty male SD rats were randomly divided into 4 groups： normal control group, asthmatic model group, desamethasone group and ligustrazine group. The asthmatic model was established by repeated inhalation of ovalbulium. The expression of NF-κB in the lung tissues was ob- served by means of immunohistochemical staining. The eosinophil （Eos） amounts and differential leukocytes counts in bronchoalveolar lavage fluid （BALF） were counted . Results （ 1 ） In the four groups, the nuclear expression of NF-κB in epithelial cells were （ 12.1 ± 2.4） %, （26.8 ± 3.6） %, （ 14.6 ± 1.9） %, （ 17.3 ± 2.6） % respectively. There was a significant increase in expression of NF-κB in the lung tissues in asthmatic model group compared with that in normal control group（ P 〈 0. 001 ）. The expression of NF-κB in ligustrazine group was much lower than that in asthmatic model group（ P 〈 0. 01 ）, There were no statistical significant difference between ligustrazine group and desamethasone group（ P 〉 0.05）. （2） The Eos amounts in BALF in lignstrazine group was much lower than that in asthmatic model group（ P 〈 0. 001 ）, There were no statistical significant difference between ligustrazine group and desamethasone group（ P 〉 0. 05）. There was a significant decrease in lymphocytes and eosinophils in BALF in ligustrazine group compared with asthmatic model group（ P 〈 0. 001 ）. Conclusion Ligustrazine can inhabit the expression of NF-κB and decrease airway inflammation in asthmatic rats.

关 键 词：川芎嗪 核因子(NF-κB) 哮喘 大鼠 

分 类 号：R284.4[医药卫生—中药学] R562.25[医药卫生—中医学]