简易气量法测试土壤过氧化氢酶活性的研究  

Measurement of Catalase Activity in Soil by Facility Gasometric Method

作　　者：罗泽娇[1] 张随成[1] 

机构地区：[1]中国地质大学环境学院,武汉430074

出　　处：《地质科技情报》2005年第4期87-90,共4页Geological Science and Technology Information

基　　金：高等学校博士点专项科研项目(20020491011)

摘　　要：通过比较现有的过氧化氢酶活性测试方法发现,气量法尽管是最简便的方法,但存在很多问题,不利于广泛使用,而简易气量法则解决了前人气量法存在的问题。利用该方法对同一个土壤样品进行的9次重复实验结果表明,格拉布斯(Grubbs)检验无剔除数据,标准偏差为1．61,变异系数为0．17,显示该方法简单、快捷、重现性好,精密度高;对20个土壤样本进行初始反应速度、最大反应速度、米氏常数测试的结果显示,它们之间的相关性显著,c(H2O2)=0．741 mol／L为最佳测试过氧化氢酶活性的底物浓度。最后对简易气量法存在的问题进行了分析。By the comparison of the current CAT activity, it is found that the gasometric method easy to use,but it could not be applied widely because of some constraints. Facility gasometric method, however, solves the problems and proves to be scientific and economic. The 9 times of test on one soil sample in the same condition show that the method does not have exceptional data to be eliminated with the standard deviation 1.61 and variation coefficient 0.17. While the tests of to soil samples on their v0 ,Vmax and Km, reflect prominent relativity among them. The best concentration of c（H2O2） is 0. 741 mol/L to measure CAT activity. The problems of this method are also discussed.

关 键 词：简易气量法 过氧化氢酶活性 最大反应速度 米氏常数 

分 类 号：X144[环境科学与工程—环境科学]