硫酸锌和蛋氨酸锌对小鼠早期生长的影响研究  

EFFECT OF ZINC SULFATE AND ZINC METHIONINE ON GROWTH AND THEIR MECHANISM IN MICE

作　　者：虞泽鹏[1] 施用晖 乐国伟 

机构地区：[1]江南大学食品学院食品营养与安全研究所,无锡214036 [2]江南大学食品安全与科学教育部重点实验室,无锡214036

出　　处：《营养学报》2005年第6期471-474,共4页Acta Nutrimenta Sinica

基　　金：科技部农业成果转化项目(No.2002730020773)

摘　　要：目的:研究硫酸锌(ZnSO4)、蛋氨酸锌(Zn-Met)两种锌补加形式对断奶小鼠早期生长的影响及其机制。方法:选取21d断奶的雄性小鼠90只(KM种)随机分3组,每组设三个重复,每重复10只。对照组饲喂玉米淀粉—酪蛋白半纯合基础日粮(含锌11.67mg/kg),ZnSO4组、Zn-Met组分别在基础日粮中添加ZnSO4、Zn-Met30mg/kg(以锌计)。饲喂10d,测定小鼠体重、血浆生长激素含量、生长激素受体基因及胰岛素样生长因子1(IGF-1)基因mRNA表达水平。结果:两种锌源均能提高小鼠体重及血清锌含量,且Zn-Met促长效果优于ZnSO4。两种锌源对生长激素水平、生长激素受体基因表达无显著影响,但均能显著提高IGF-ImRNA表达水平;Zn-Met处理小鼠IGF-ImRNA表达量显著高于ZnSO4处理。结论:ZnSO4和Zn-Met对生长激素水平、生长激素受体基因表达无影响,但均上调IGF-ImRNA表达量。Zn-Met较ZnSO4更能有效提高小鼠体重和上调IGF-ImRNA表达。Objective： To investigate the effect of zinc sulfate and zinc methionine on growth and their possible regulating mechanism in mice. Method： Ninety male KM mice were randomly divided into three groups. The control group was fed on basal diet containing zinc of 11. 67 mg/kg 10d. The ZnSO4 group and Zn-Met group were fed on the diets supplemented with ZnSO4 or Zn-Met at 30 mg/kg（on the basis of Zn） for 10 d. Initial and final body weight, serum zinc concentration, growth hormone （GH）, the levels of growth hormone receptor （GHR） and insulin like growth factor 1 （IGF-I） mRNA were determined. Results： Both ZnSO4 and Zn-Met enhanced body weight and serum zinc concentration of mice, Zn-Met more effectively than ZnSO4 for body weight. Both forms of zinc had no effect on GH and the expression of GHR mRNA , but both up-regulated the expression of IGF-1 mRNA. As compared to ZnSO4, Zn-Met enhanced the level of IGF-1 mRNA significantly. Conclusion： Both ZnSO4 and Zn-Met had no effect on GH and the expression of GHR Mrna, but enhanced the expression of IGF-1 mRNA. Zn-Met enhanced the body weight gain and up-regulated IGF- 1 mRNA expression more effectively than ZnSO4.

关 键 词：硫酸锌 蛋氨酸锌 生长 生长激素受体 胰岛素样生长因子 

分 类 号：R151[医药卫生—营养与食品卫生学]