粉防己多糖的组成及其清除活性氧自由基的作用(英文)  

Study on Chemical Composition and Antioxygenation of Polysaccharide from Stephania Tetrandra S.Moore

作　　者：甄攀[1] 梁惠花[1] 张万明[1] 王治宝[1] 

机构地区：[1]河北北方学院化学教研室,河北张家口075000

出　　处：《河北北方学院学报（医学版）》2005年第5期6-9,共4页Journal of Hebei North University:Medical Edition

摘　　要：目的:提取并纯化粉防己多糖(SMP),确定其组成,研究其清除活性氧自由基的作用。方法:利用水提醇沉得到粉防己粗多糖,再经DEAE纤维素和聚酰胺纯制。利用Fenton反应研究SMP清除.OH的作用;利用邻苯三酚自氧化法研究SMP清除.O2-的作用。结果:纸色谱显示SMP是由葡萄糖组成的匀多糖;SMP清除.OH的能力较弱;清除.O2-时的效果稍强,IC50为1956.1μg/ml。结论:粉防己多糖为首次提取制得,有一定的清除羟自由基和超氧阴离子自由基的作用。Objective： To extract and purify Polysaccharide from Stephania tetrandra S. Moore（SMP）, study on its chemical composition and the effect on scavenging active oxygen. Methods： The Polysaccharide was extracted by water and precipitated by ethanol. It was further purified by DEAE-cellulose and polyamide. Study Polysaccharide scavenging effect on·OH with Fenton reaction. Study on Polysaccharide scavenging effect on ·O2^- with Pyrogallol Autoxidation Method. Results： The paper chromatography showed that SMP was composed of glucose. The scavenging effect of SMP on·OH was displeased. The IC50 of SMP scavenging ·O2^- was 1956. μg/ml. Conclusion： This Polysaccharide was isolated from Stephania tetrandra S. Moore for the first time, which had certain effect on scavenging ·OH and ·O2^-.

关 键 词：粉防己 多糖 抗氧化 羟自由基 超氧阴离子自由基 

分 类 号：R914[医药卫生—药物化学]