机械牵张对缺血缺氧心肌细胞微管的影响  

Effects of Mechanical Stretch on Myocardial Microtubules under Ischemia and Hypoxia in Vitro

作　　者：李晓东[1] 杨建民[1] 黄跃生[2] 

机构地区：[1]解放军白求恩国际和平医院烧伤整形科,石家庄050082 [2]第三军医大学西南医院全军烧伤研究所,重庆400038

出　　处：《华北国防医药》2005年第6期381-383,F0002,共4页Medical Journal of Beijing Military Region

基　　金：国家杰出青年科学基金(No.30125040);国家重点基础研究发展规划项目(No.G199054202);创伤烧伤复合伤国家重点实验室开放课题基金(No.200304)

摘　　要：目的:探讨机械牵张对缺血缺氧心肌细胞微管的影响。方法:建立离体培养的SD乳鼠心肌细胞机械牵张模型,施加无糖缺氧刺激因素模拟烧伤早期缺血缺氧损害,实验分为10%牵张组(S组)、缺血缺氧组(I组)及10%牵张+缺血缺氧组(IS组),分别在刺激前和后1、3、6、12小时4个时相点进行观察,采用免疫荧光染色观察微管的变化,并用免疫蛋白印迹检测-βtubu lin含量变化。结果:3小时后S组微管呈现增强趋势,I组微管有明显破坏,可见大量断裂微管分布于核周,IS组微管破坏严重,无聚合状态微管存在,细胞完整性受损。刺激后S组β-tubu lin含量逐渐增多,I组则减少,IS组减少显著。12小时后IS组与其他2组差异显著(P<0.01)。结论:机械牵张加重了缺血缺氧对心肌细胞微管的破坏,可能是“休克心”发生发展的重要原因之一。Objective：To investigate the effects of mechanical stretch on myocardial microtubule-exposed to ischemia and hypoxia in vitro. Methods ：SD neonate rat myocardial cells were cultured in ：vitro and stimulated by static mechanical stretch under ischemia and hypoxia exposure. The early ischemic and hypoxia injury in burn was simulated. The alterations of microtubule and tubulin were observed by laser confocal microscopy （LCM） and Western blotting at 1,3,6 and 12 hour after the exposure. Experimental protocol was composed of three different groups：10% stretched group （Group S） ,ischemia and hypoxia group （Group Ⅰ） and 10% stretched plus ischemia and hypoxia group （Group IS）. Results：After being exposed to ischemia and hypoxia for 3 hours,microtubule was increased in Group S,while it was damaged severely and broken with large amount of broken microtubules around the nucleus;in Group IS,they were severely destructed and no polymerized microtubules could be noted ;the integrity of myocardial cells was damaged. After the exposure, content of β-tubulin was increased gradually in Group S whereas it was decreased in Group I and significantly decreased in Group IS with a significant difference from the other two groups 12 hours after the exposure （P 〈 0. 01 ）. Conclusions： Mechanical stretch worsen microtubule damage caused by ischemia and hypoxia. This might be regarded as one of the important etiological factors of “ shock heart”.

关 键 词：心肌缺血 缺氧 微管 机械牵张 乳鼠 SD 

分 类 号：R361.3[医药卫生—病理学]