C-MYC癌基因与消化系统肿瘤关系的研究  

Research on C-MYC oncogene amplification with stomach, colon carcinomas andhepatoma

作　　者：卞隽 方艳秋[2] 谭岩[2] 段秀梅[2] 许淑芬[2] 刘力华[2] 姜艳芳[2] 赵平伟[3] 

机构地区：[1]吉林省肿瘤医院,130012 [2]吉林大学第一医院中心实验室 [3]吉林大学第四医院肝胆外科

出　　处：《中国妇幼保健》2005年第24期3314-3316,共3页Maternal and Child Health Care of China

基　　金：吉林省科技厅国际合作项目(编号:20050703-6);吉林省科技厅重点项目(编号:20040401-5);吉林省科技厅国际合作项目(编号:20040707-2);2004年长春市科技局计划项目(编号:2004218);高等学校博士学科点专项科研基金(编号:20020183017)

摘　　要：目的:研究消化系统肿瘤中的C-MYC基因扩增的情况。方法:用聚合酶链式反应(PCR)测定84例老年患者恶性肿瘤中的C-MYC基因,通过激光密度扫描仪对EB染色后的琼脂糖凝胶进行积分光密度分析。结果:正常组织无C-MYC扩增,C-MYC/N+L-MYC比值95%可信区间为0.0869～0.6257,胃癌组织C-MYC基因扩增阳性率为69.5%,直肠癌组织的C-MYC基因扩增阳性率为62.2%,肝癌组织C-MYC基因扩增阳性率为55.6%,145例肿瘤组织C-MYC扩增总阳性率为65.5%。结论:癌组织C-MYC扩增高于正常组织,在不同消化系统肿瘤组织中扩增程度不同,与细胞潜在的增殖能力成正相关,对消化系统肿瘤的发生、发展及预后,特别是指导临床基因治疗提供有用的资料。Objective： To study the amplification of C - MYC gene in gastroenterological careinoma Methods： 145 cases with different tumors were detected by polymerase chain reaction （PCR）, and the results staining with EB were analysised by integrating density. Results： The amplification of C - MYC gene was not found in 32 normal controls, and the average density of C - MYC/N ＋ L - MYC was O, 086 9 -0. 625 7. The positive rate of C - MYC amplification in 45 colon carcinoma was 62. 2% , the positive rate of C - MYC amplification in 82 stomach cancers renal was 69. 5% , the positive rate of C - MYC amplification in 18 hepatomas renal was 55.6% , the positive rate was 65. 5% in the total 145 cases with different cancers. Conclusion： C -MYC gene was related with cell growth, differentiation and cancer, the detecting C - MYC amplification was useful for gene therapy with antisense oligonucleotide to C - MYC.

关 键 词：癌基因 C—MYC 胃癌 直肠癌 肝癌 

分 类 号：R735[医药卫生—肿瘤]