低剂量辐射对荷人胶质瘤(U251)裸小鼠移植瘤细胞凋亡相关基因蛋白Bcl-2表达的影响  

e radiation on expression of apoptosis-related gene protein Bcl-2 in transplantation tumor tissues of human glioma(U251)-bearing nude mice

作　　者：纪莉[1] 姜宏宇 关井会[1] 

机构地区：[1]吉林大学第一临床医学院老年病科,吉林长春130021

出　　处：《中国老年学杂志》2005年第12期1496-1497,共2页Chinese Journal of Gerontology

基　　金：吉林省科技发展计划资助课题(200505183)

摘　　要：目的研究低剂量辐射对荷人胶质瘤(U251)裸小鼠移植瘤细胞凋亡相关基因蛋白Bcl-2表达的影响。方法对荷人胶质瘤裸小鼠进行全身深部X射线照射,采用免疫组化技术检测荷人胶质瘤裸小鼠移植瘤组织的Bcl-2蛋白表达水平。结果D1(75 mGy)照射后,胶质瘤细胞的Bcl-2蛋白表达呈下降趋势,与假照组(0 mGy)比,无显著差异(P>0.05);D2(4 Gy)组、D1+D2组的Bcl-2蛋白表达明显减少,与假照组(0 mGy)比有统计学差异(P<0.05);而D1+D2组与D2组比较,有统计学差异(P<0.05),以D1+D2组Bcl-2蛋白的表达减少更为显著。结论低剂量辐射在一定程度上可能下调了胶质瘤细胞的Bcl-2蛋白的表达,同时对其后的大剂量辐射有协同作用。Objective To study the effect of low dose radiation on expression of apoptosis-related gene protein Bcl-2 in transplantation tumor tissues of human glioma （ U251 ） -bearing nude mice. Methods Tumor （ U251 ） -bearing nude mice radiated by whole body X ray were used to detect the expression of apoptosis-related gene protein Bcl-2 of transplantation tumor tissues by immunohistochemical method. Results The expression of Bcl-2 protein showed slight but not significant decrease in D1 （75 mGy） group comparing to that of control （ P 〉 0. 05 ）. While in D2 （4 Gy） and D1 ＋ D2 groups, the expression of Bcl-2 protein significantly decreased comparing to that of controls （ P 〈 0. 05 ）, that of D1 ＋ D2 group was more obviously. Conclusions Low dose radiation could down-regulate the expression of Bcl-2 protein to some extent, and maybe have synergistic effect with high dose radiation thenceforth.

关 键 词：低剂量辐射 人胶质瘤(U251) 裸小鼠 凋亡 免疫组化 基因 

分 类 号：R730.264[医药卫生—肿瘤]