电化学发光在基因检测中的应用  

The Application of Eelectrochemiluminescence in Gene Detection

作　　者：唐亚兵[1] 唐永红[1] 

机构地区：[1]华南师范大学激光生命科学研究所

出　　处：《激光生物学报》2005年第6期461-465,共5页Acta Laser Biology Sinica

基　　金：国家自然科学基金项目(60378043;30470494);广东省自然科学基金项目(015012;04010394)

摘　　要：电化学发光基因检测是把电化学发光的高灵敏性和传统分子生物学方法的稳定性结合于一体的一种新型的基因检测技术。与传统的基因检测方法相比,它具有无放射性危害、高灵敏度、操作简便等优点。近年被广泛地应用于核酸序列分析,基因突变分析,遗传病、转基因物种、病毒、微生物等的检测。本文概述了电化学发光的基本原理以及传统的基因检测技术,详细地介绍了电化学发光在当前基因检测中的应用现状,并对其前景作了展望。Electrochemiluminescence technique has became a new gene detection method. It combined the sensitivity of electrochemiluminescence with the stability of traditional biology detection method. Compared with traditional gene detection technique, it is a non-radioactive hazard, it has high sensitivity and simplicity. It has been widely applied in gene analysis, detection of gene mutation and genetic disease. This paper introduced the basic principle of electrochemiluminescence and its application in gene detection. Ultimately, the bright future of electrochemiluminescence application in gene detection is prospected.

关 键 词：电化学发光 基因检测 检测方法 

分 类 号：Q503[生物学—生物化学] Q78