发色底物法测定注射用降纤酶中类凝血酶的含量  

Determination of thrombin-like in defibrase for injection by chromogenic substrate

作　　者：雷丹青[1] 刘绵林[1] 周先丽[1] 

机构地区：[1]广西医科大学蛇毒研究所,广西南宁530021

出　　处：《中国现代应用药学》2005年第6期482-483,共2页Chinese Journal of Modern Applied Pharmacy

基　　金：广西壮族自治区自然科学基金资助项目(0342002-4)

摘　　要：目的建立发色底物法测定注射用降纤酶类凝血酶的方法.方法以Be-Phe-Val-Arg-PNA为发色底物,检测波长405nm.结果降纤酶在2～10单位/mL范围内呈良好的线性关系,r=0.9993(n=5).结论本法简便,准确,专属性强,适用于产品的质量控制.OBJECTIVE A chromogenic substrate method was developed for the determination of thrombin-like in defibrase for injection. METHODS Chromogenic substrate was Be-Pbe-Val-Arg-PNA. The detection was made at 405nm. RESULTS The calibration curve of thrombin-like had good linear relationship in the range of 2 ～10unit/mL （ r = 0. 9993, n = 5）. CONCLUSION The developed chromogenic substrate method is simple,accurate and specific, and could be used as the quality control for thrombin-like in defibrase for injection.

关 键 词：降纤酶 类凝血酶 发色底物 含量测定 

分 类 号：P027.2[天文地球]