β射线对NIH3T3成纤维细胞细胞周期的影响及牛磺酸的干预作用  

Effect of β-radioation on Cell Cycle of NIH3T3 Fibroblast and Interference of Taurine

作　　者：范雁[1] 徐爱华[1] 周迎会[1] 陈美[1] 吴士良[1] 

机构地区：[1]苏州大学医学院生化与分子生物学教研室,江苏苏州215123

出　　处：《江苏大学学报（医学版）》2005年第6期470-472,共3页Journal of Jiangsu University：Medicine Edition

基　　金：国防科工委国防预研基金资助项目(Y69200-04);江苏省高校自然科学研究项目(01KJB310001)

摘　　要：目的:观察β射线对NIH3T3成纤维细胞周期的影响,探讨β放射损伤的机制及牛磺酸(taurine)的辐射防护作用.方法:以不同剂量的高能电子射线模拟β射线照射NIH3T3细胞,加药组分别为(50 mg/L、100 mg/L、200mg/L)选择15 Gy照射剂量,采用流式细胞术检测各组细胞的细胞周期率.结果:β辐射后细胞发生G2期阻滞,且与照射剂量呈正相关(P≤0.01).在0～15 Gy剂量范围内,β辐照12 h后未观察到细胞发生凋亡.加入牛磺酸后,G2期受阻现象得到缓和,G2/M期百分率与药物剂量呈负相关(P≤0.01).结论:β辐射后NIH3T3细胞属于G2期延迟细胞,牛磺酸可通过一定机制使受损的细胞不能通过G1期,从而有助于加强对细胞受损DNA的修复,起到辐射防护的作用.Objective： To observe the effects of β-radioation on cell cycle of NIH3T3 fibroblasts and to study the mechanism of β-radioation＇s damage and the radioprotective effect of Taurine. Method： NIH3T3 cells were irradiated with different dosages of high energy electronic ray simulated β-rays, Tau groups （50 mg/L,100 mg/L,200 mg/L）were irradiated with 15 Gy dosage. The cell cycle of each group was determined by flow cytometry within 12h. Result： After β-radioation, NIH3T3 cells showed the G2/M phase was delayed, and it was related to the dosage of irradioation（ P≤0. 01 ）. At the range of 0 - 15 Gy, apoptosis can＇t be achieved within 12h after irradioation. Tau can alleviate the delay of G2/M phase, the percentage of G2/M was negatively relative to the dosage of Tau（ P≤0. 01 ）. Conclusion： NIH3T3 cells achieved the delay of G2/M phase after β-radioation, Tau can inhibit NIH3T3 cells to pass through G1 phase and improved the ability of repairing DNA by extension G1 phase in cell cycle, so Tau has radioprotective activity on cell levels.

关 键 词：β辐照 NIH3T3细胞 细胞周期 牛磺酸 

分 类 号：R811.3[医药卫生—放射医学]