Annexin V的^(125)I标记及其在正常小鼠体内的分布  

Labeling of Annexin V With ^(125)I and Its Biodistribution in Mice

作　　者：靳彩玲[1] 许玉杰[1] 范我[1] 华子春[2] 胡明江[1] 王道锦[1] 朱然[1] 张友九[1] 

机构地区：[1]苏州大学放射医学与公共卫生学院,江苏苏州215007 [2]南京大学医药生物技术国家重点实验室,江苏南京210093

出　　处：《同位素》2005年第4期225-228,共4页Journal of Isotopes

基　　金：江苏省高校自然科学基金(04KJB350130);江苏省核医学与生物技术重点实验室开放基金(KJS01010)资助项目

摘　　要：采用Iodogen法对Annexin V进行了125I标记,并观察了其在正常小鼠体内的分布情况。标记结果显示,125I-Annexin V标记率达94.9%,纯化后放化纯度达99%;室温放置72 h后,放化纯度仍保持在92%以上,表明其体外稳定性较好。生物分布结果显示,125I-Annexin V在肾脏中放射活性最高,其次为血液、肝脏、心、肺、脾;脑不吸收125I-Annexin V;肌肉、骨骼组织摄取亦较少;各组织、器官放射性摄取在1 h内除血液下降稍慢外,其余均有明显下降。表明125I-Annexin V适合用作核医学诊断试剂。Annexin V is labeled with ^125I by using Iodogen method and the biodistribution of which in mice is investigated. The labeling yield of ^125I-Annexin V is 94.9% and its radiochemical purity is over 99% after parification. The radioactivity uptake is the highest in kidney, followed in order by blood, liver, heart, lung and spleen;no uptake in brain; the lower in muscle and bone;The uptake of the selected tissues is decreased rapidly at 1 hour after injection except blood. The results show that it is a excellent imaging agent for diagnoses.

关 键 词：^125I标记 ANNEXIN V 生物分布 

分 类 号：TL92[核科学技术—核燃料循环与材料] R817.3[医药卫生—影像医学与核医学]