福建汉族急性淋巴细胞白血病及慢性粒细胞白血病HLA-DRB1基因分析  

Analysis of HLA-DRB1 alleles in Fujian Chinese patients with acute lymphoblastic leukemia and chronic myelogenous leukemia

作　　者：金静君[1] 苏东辉[2] 陈志哲[3] 胡洁[1] 薛士杰[1] 何萍[1] 赖力[1] 

机构地区：[1]福建省医学科学研究所免疫学研究室,350001 [2]福建医科大学基础医学院免疫学系,350004 [3]福建医科大学附属协和医院血液病研究所,350001

出　　处：《福建医药杂志》2005年第6期126-128,共3页Fujian Medical Journal

基　　金：福建省医学创新课题(项目编号:2003CX14)

摘　　要：目的探讨HLADRB1基因多态性与急性淋巴细胞白血病(ALL)、慢性粒细胞白血病(CML)之间的关联。方法用聚合酶链反应序列特异引物(PCRSSP)方法,对32例ALL、33例CML患者和104例健康志愿者的HLADRB1基因进行分型并分析HLADRB1基因的分布。结果与正常对照组相比,ALL患者的HLADRB106的基因频率明显增高,为15.63%,RR=3.84,χ2=8.01,P<0.001;CML患者的HLADRB109的基因频率明显增高,为24.24%,RR=3.14,χ2=7.82,P<0.01,结论HLADRB106与急性淋巴细胞白血病有关联,HLADRB109与慢性粒细胞白血病有关联。Objective To clarify the association of HLA-DRB1 alleles in patients with acute lymphoblastic leukemia （ALL） and chronic myelogenous leukemia （CML）. Methods HLA-DRB1 allele frequencies in 32 Chinese patients with ALL, 33 Chinese patients with CML and 104 healthy Chinese individuals were examined with polymerase chain reaction-specific sequence primers （PCR-SSP） methods. Results The gene frequency of HLA-DRB1 ＊ 06 in all patients is significantly higher than that in healthy controls （15.63% vs 5. 29%, P〈0. 001）. The relative risk of HLA-DRB1 ＊ 06 to ALL is 3.84. The gene frequency of HLA-DRB1 ＊ 09 in CML patients is significantly higher than that in healthy controls （24. 24% vs 11.54%, P〈 0. 01）. The relative risk of HLA-DRB1 ＊ 06 to CML is 3.14. The present results suggest positive association of HLA-DRB1 06 with ALL, and HLA-DRB1 ＊ 09 with CML respectively. Conclusion HLA-DRB1 ＊ 06 and HLA-DRB1 ＊ 09 alleles seem to contribute to the genetic susceptibility for patients with ALL and CML respectively.

关 键 词：急性淋巴细胞白血病 慢性粒细胞白血病 HLA-DRB1 基因关联 聚合酶链反应-序列特异引物 

分 类 号：R733.7[医药卫生—肿瘤] Q78[医药卫生—临床医学]