DDT降解菌株DB-1的分离、系统发育及降解特性  

Isolation, phylogenesis and degradation characters of DDT-degrading bacterium strain DB-1.

作　　者：张明星[1] 洪青[1] 何健[1] 许育新[1] 李顺鹏[1] 

机构地区：[1]南京农业大学生命科学院农业部农业环境微生物工程重点开放实验室,江苏南京210095

出　　处：《中国环境科学》2005年第6期674-677,共4页China Environmental Science

基　　金：国家"863"项目(2004AA246070;2004AA214102);国家自然科学基金资助项目(30400013);江苏省科技厅项目(BG2005322)

摘　　要：从农药厂下水道污泥中分离、筛选到1株能够在好氧条件下降解DDT的细菌菌株DB-1,根据表型特征、生理生化特性及16S rDNA序列的系统发育分析,将菌株DB-1初步鉴定为鞘氨醇单胞菌属(Sphingomonas sp).该菌株能在含酵母膏(40mg/L)的DDT(40mg/L)无机盐液体培养基中降解DDT,10d降解率达到83.6%,菌株DB-1在25～30℃长势较好,最适生长pH值为8.0.A bacterium strain DB- 1 capable of degrading DDT under the aerobic condition was isolated and screened from the sewer sludge of a pestcide factory. Based on the phenotype, physiological and biochemical characteristics, and the phylogenetic analysis of 16S rDNA sequence, the strain DB-1 was identified preliminarily as Sphingomonas sp. This strain could degrade DDT in inorganic salt liquor culture base of DDT （40mg/L） containing yeast cream （40mg/L）, with degradation efficiency reaching 83.6% in 10 days. The strain DB-1 growed better at 25-30℃, with most adaptable pH value of 8.0.

关 键 词：DDT 农药残留 生物降解 系统发育分析 

分 类 号：X172[环境科学与工程—环境科学]