人大肠癌原代培养体外药敏试验的研究  

Evaluation of chemosensitivity of human colorectal cancer determined by APT-TCA assay in vitro

作　　者：李艳芬[1] 田海梅[1] 周志祥[2] 曹冬艳[1] 吴健雄[2] 荣维淇[2] 刘毅[1] 李茉[1] 王海鹏[2] 王小兵[1] 刘朝阳[1] 梁智[1] 程冬婉[1] 张伟[1] 

机构地区：[1]中国医学科学院中国协和医科大学肿瘤医院肿瘤所生物检测中心,北京100021 [2]中国医学科学院中国协和医科大学肿瘤医院腹部外科,北京100021

出　　处：《中华检验医学杂志》2005年第12期1231-1234,共4页Chinese Journal of Laboratory Medicine

摘　　要：目的探讨用生物荧光肿瘤体外药敏检测技术(ATPTCA)研究大肠癌药敏的异质性和个体化疗的可行性。方法用ATPTCA技术检测50份大肠癌标本对16种抗癌药单药或联合用药的敏感性。结果不同标本的药物敏感性存在着明显差异,单药中最有效的药物依次为长春瑞滨、羟基喜树碱、氟尿嘧啶和紫杉醇,敏感率分别为34.1%、31.6%、27.6%和24.3%。最有效联合用药方案是氟尿嘧啶+丝裂霉素+阿糖胞苷,敏感率为100%(11/11份),其次是氟尿嘧啶+顺铂+阿霉素和健择+顺铂。结论大肠癌对抗癌药物的敏感程度普遍较低,且存在明显异质性。试验结果与临床治疗经验比较一致,ATPTCA可用于为大肠癌患者选择合适的化疗药物。Objective To investigate the heterogeneity of chemosensitivity in colorectal cancer using an ATP-tumor chemosensitivity assay （ATP-TCA） and the feasibility of individual chemotherapy. Methods An ATP-TCA were used to determine the effect of 16 single or combined cytotoxic drugs in surgical specimens from 50 patients with colorectal cancer. Results There were considerable differences in chemosensitivity between individuals. The most active single drugs in the assay was identified as Navelbine, Hydroxycamptothecin, 5-Fluorouracil and Paclitaxel; 34. 1%, 31.6%, 27.6% and 24. 3% of specimens showed sensitivity to them, respectively. 5-Fluorouracil ＋ Mitomycin ＋ Aytarabine was found to be the most effective combination, for 100% （ 11/11 ） specimens were sensitive to this regimen. 5-Fluorouracil ＋ Cisplatin ＋ Adriamycin and Gemcitabine ＋ Cisplatin were moderately active regimens. Conclusions There was the heterogeneity of the in vitro chemosensitivity in colorectal cancer. The use of the ATP-TCA provides a method of selecting appropriate anti-cancer drugs in colorectal cancer.

关 键 词：生物荧光肿瘤药敏检测技术 大肠癌 药物敏感性 原代培养 

分 类 号：R735.34[医药卫生—肿瘤]