壳聚糖固定化酵母蔗糖酶的研究  

Study on the Properties of Chitsan-immoblized Yeast Sucrase

作　　者：马中良[1] 李艳利[2] 焦吉祥[1] 邹媛媛[1] 鲍真真[1] 王旻[1] 

机构地区：[1]中国药科大学生命科学与技术学院,江苏南京210009 [2]南京医科大学生化与分子生物学系,江苏南京210029

出　　处：《药物生物技术》2005年第6期379-382,共4页Pharmaceutical Biotechnology

基　　金：江苏省博士后基金

摘　　要：用壳聚糖作吸附剂、戊二醛作交联剂,对酵母蔗糖酶进行固定化研究,同时用琼脂糖固定化的蔗糖酶和游离酶与其进行比较。结果表明,壳聚糖固定化蔗糖酶贮藏稳定性、对变性剂以及对温度的耐受性均明显优越,而Km值与琼脂糖固定化酶相当,但比游离酶明显增高,酶的最适pH三者相近。研究表明,壳聚糖固定化酶所需的戊二醛浓度为0.6%,交联时间不低于6 h。固定化蔗糖酶对变性剂(乙醇、脲)的耐受力明显高于游离酶。Yeast sucrase immobilized to chitosan with glutaric dialdehyde as cross-linking agent was studied, while agrose-immobilized and free enzymes were as control. The results showed the stability of chitosan-immobilized enzyme increased much more than that of agarose-immobilized enzyme or free enzyme. The Michaelis-Menten Kinetics of chitosan-immobilized enzyme was equal to that of agarose-immobilized enzyme, but much higher than that of free enzyme. The optimum pH of these enzymes was almost the same. In preparation chitosan-immobilized enzyme, 0. 6% glutaric dialdehyde and more than 6 hr crosslinking were required. To free enzyme, chitosan-immobilized enzyme was more stable in ancohol or urea.

关 键 词：壳聚糖 固定化技术 酵母蔗糖酶 甲壳素 葡萄糖 药物生产 

分 类 号：R915[医药卫生—微生物与生化药学]