桩蛋白和突变型p53蛋白在肺癌组织中的表达及其临床意义  

Expression and Clinical Significance of Paxillin and mt-p53 in Human Lung Cancer Tissues

作　　者：彭品贤[1] 张鑫[1] 吕嘉春[2] 梁建辉[1] 吴建军[2] 曾波航[1] 

机构地区：[1]广州医学院第二附属医院,广东广州510260 [2]广州医学院化学致癌研究所,广东广州510182

出　　处：《国际医药卫生导报》2005年第24期4-7,共4页International Medicine and Health Guidance News

基　　金：国家自然科学基金资助项目(30200235;30371196);广东省医学科研项目(A2003320);广州医学院科学研究项目(02-K-33)

摘　　要：目的探讨桩蛋白(Paxillin)、突变型p53蛋白(mutant-p53,mt-p53)在人肺癌组织中表达及其临床意义。方法应用免疫组织化学SP方法检测120例肺癌、80例癌旁无癌浸润肺组织、53例正常肺组织的Paxillin和mt-p53蛋白的表达。结果肺癌、癌旁无癌浸润肺组织和正常肺组织Paxillin的阳性表达率分别为39.1%(47/120)、81.3%(65/80)、83.3%(44/53),三者的差异有统计学意义,P<0.01。mt-p53蛋白阳性表达率分别为54.2%(65/120)、7.5%(6/80)、0.0%(0/53),三者的阳性表达差异也有统计学意义,P<0.01。肺癌组织中Paxillin的阳性表达与肺癌的临床病理分期、肺癌分化程度、淋巴结转移有关,P<0.05。mt-p53蛋白阳性表达率与肺癌的临床分期无显著相关性(P>0.05),与肺癌的恶性程度及淋巴转移呈正相关(P<0.05)。Paxillin、mt-p53阳性表达率与肺癌术后3年生存率之间关系,无统计学意义(P>0.05)。肺癌Paxillin与mt-p53的阳性表达呈负相关,P<0.05。结论检测肺癌组织中Paxillin、mt-p53对判断肺癌的恶性程度、淋巴转移趋势具有重要价值。Objective To study the expression and prognostic value of Paxillin and mutant-p53 （mt-p53） in human lung cancer. Methods SP immunohistochemical staining was applied to detected the expressions of Paxillin and mt-p53 in 120 cases of human primary lung cancer＇s tumor tissues, 80 cases of adjacent lung tissues without carcinoma and 53 cases of normal lung tissues. Results The positive staining rate of Paxillin in primary lesion, adjacent lung tissues without carcinoma and normal lung tissues was 39.1%（47/ 120）, 81.3% （65/80） and 83.3% （44/53） respectively. The difference was high significant, P〈0.01. Positive expression rate of mr-p53 protein in the three places was 54.2% （65/120）, 7.5% （6/80） and 0. 0% （0/53） respectively. The difference was significant too, P〈0.01. The positive expression rates of Paxillin in lung cancer tissues were associated with the lung cancer patient＇s histological grades, clinicopathological stages and lymph node invading , P〈0. 05. The relationship between positive expression of mt-p53 in lung cancer tissues clinicopathological stages and lymph node invading was associated, P〈0. 05. There was no difference between positive expression of mt-p53 in lung cancer tissues and the histological grades, P〉0. 05. The positive expression of Paxillin and mt-p53 in lung cancer tissues and postoperative prognosis of 3 years survival was not associated. P〉0. 05. But there was inverse correlation between the paxillin positive staining rate and mutant-p53 staining, P〈0.05. Conclusions The positive expression of Paxillin and mt-p53 protein might associated with lung cancer metastasis and the expression of them can used as valuable biomarkers to evaluate lung cancer metastasis.

关 键 词：肺肿瘤 免疫组织化学 桩蛋白 突变型P53蛋白 

分 类 号：R734.2[医药卫生—肿瘤]