水蛭桃仁汤对肝纤维化小鼠肝细胞凋亡的影响  

Effect of Decoction of leech and peach kernel on hepatocyte apoptosis of mice with liver fibrosis

作　　者：陈姝[1] 陈建明[1] 

机构地区：[1]武汉科技大学医学院,湖北武汉430080

出　　处：《现代实用医学》2005年第12期737-738,748,共3页Modern Practical Medicine

摘　　要：目的观察水蛭桃仁汤对肝纤维化小鼠肝细胞凋亡的影响,探讨其对肝纤维化是否具有治疗作用及其作用机制。方法制备小鼠血吸虫性肝纤维化模型,给予水蛭桃仁汤灌胃治疗,采用免疫组化细胞凋亡染色(Tunel)结合形态计量分析,观察小鼠肝细胞凋亡的形态和数量改变,并做组织病理学检测。结果经图像分析,药物防治组的肝纤维化程度显著降低,细胞凋亡数量显著减少(均P<0.01)。结论水蛭桃仁汤可抑制纤维化小鼠肝细胞的凋亡,这可能是其抗肝纤维化的机制之一。Objective To study the effects of Decoction of leech and peach kemel on hepatocyte Apoptosis of mice with liver fibrosis and investigate the possible mechanisms in protection of liver fibrosis. Methods The mice fibrosis model was induced by the infection of Schistosoma Japonieum . At the same time, the treatment group was treated with Decoction of leech and peach kernel, and immunohistochemieal and stereologieal analyses were used to observed the morphological and count change of hepatocyte apoptosis. Results By an image analysis system, the number of hepatocyte apoptosis of the treatment group was more less than that of the control group（ P 〈 0.01 ）. Conclusion Decoction of leech and peach kernel can inhibite apoptosis of hepatocyte and it may be one of the anti-hepatic fibrosis mechanisms.

关 键 词：血吸虫病 肝硬化 中草药 肝细胞 细胞凋亡 小鼠 

分 类 号：R575.2[医药卫生—消化系统] R289.5[医药卫生—内科学]