MMP-2与TIMP-2在大肠癌中的表达  

Expression of MMP-2 and TIMP-2 in carcinoma of large intestine

作　　者：穆林菁[1] 楼善贤[1] 朱寿田[1] 徐庆才[1] 

机构地区：[1]浙江省金华市中心医院病理科,金华321000

出　　处：《实用肿瘤学杂志》2005年第6期408-410,共3页Practical Oncology Journal

摘　　要：目的探讨大肠癌组织中基质金属蛋白酶(Matrix Meralloproteinases,MMPS)和金属蛋白酶组织抑制物(Tissue Inhibitors of Meralloproteinases,TIMPS)基因表达与肿瘤发生,浸润深度,转移及预后之间的关系。方法采用免疫组化S-P法对98例大肠癌组织及40例大肠良性病变组织进行MMP-2和TIMP-2的检测。结果98例大肠癌组织中MMP-2、TIMP-2的表达分别为75.51%(74/98)、57.14%(56/98),而在癌旁组织及良性病变中大部分不着色,少部分有微弱着色,二者的表达与肿瘤的发生明显相关;MMP-2的高表达与肿瘤的浸润深度、淋巴结转移及临床预后均呈明显的正相关(P<0.05),TIMP-2的高表达与淋巴结转移及预后呈明显的负相关(P<0.05)。结论大肠癌组织中MMP-2的高表达预示着病人预后更差,评价MMP-2和TIMP-2之间的平衡比单独采用MMP-2指标更有利于了解肿瘤的进展及预后。Objective To study the presence and celluar location of matrix metalloproteinase MMP - 2 and tissue inhibitor of metalloproteinase TIMP - 2 in carcinoma of large intestine. Methods The expression of MMP- 2 and TIMP - 2 was detected using immunohistochemistry on tissue sections from 98 carcinoma of large intestine and 40 polyp or adenoma of large intestine. Results Immunestaining of MMP - 2 and TIMP - 2 ill carcinoma of large intestine tissue was observed in 75.51% （74/98） and 57.41% （56/98） respectivly. Weaker immunostaining, of the two antibodies was observed in only a few epithelia in the surrounding background large intestine tissue and polyp or adenoma. The presence of MMP - 2 and TIMP - 2 was significantly greater in carcinoma of large intestine tissues than that of all other large intestine tissues. The high level of MMP - 2 was associated with more lymph node metastasis and increased recurrence of carcinoma of large intestine （ P 〈 0.05）. The high level of TIMP - 2 correlate inversely with the metastasis and recurrence （ P 〈 0.05）. Conclusion The study suggests that high expression of MMP - 2 in carcivoma of large intestine tissue way be associated with an unfavorable prognosis while high expression of TIMP- 2 may correlate with longer disease free survival. The ratio of MMP- 2 and TIMP- 2 provides more predictive information and may be a strong prognostic indicator of carcinoma of large intestine. Induction of TIMP - 2 expression can be considered as a alternate therapy regiment to protect the patients from tumor recurrence and metastasis.

关 键 词：大肠癌 基质金属蛋白酶 金属蛋白酶组织抑制物 免疫组织化学 

分 类 号：R735.34[医药卫生—肿瘤]