流式细胞仪测定成人急性白血病患者DNA含量的临床意义  

Clinical significance of DNA content assay in adult acute leukemia by flow cytometry

作　　者：梁英民[1] 张盈华[1] 林榕 黄昌亮[1] 邓中荣[1] 郝淼旺[1] 吴绒丽[1] 

机构地区：[1]第四军医大学唐都医院血液科 中心实验室

出　　处：《第四军医大学学报》1996年第3期206-208,共3页Journal of the Fourth Military Medical University

摘　　要：目的：为探讨急性白血病（ＡＬ）患者骨髓细胞的周期分布、非整倍体，及其与临床疗效的关系．方法：作者应用流式细胞仪（ＦＣＭ）测定了５７例ＡＬ患者的骨髓细胞ＤＮＡ含量．结果：不同类型ＡＬ患者骨髓细胞Ｓ％均明显低于正常对照和缓解后患者．急性早幼粒细胞白血病（Ｍ３）Ｓ％最低；ＡＬ患者骨髓细胞ＤＮＡ非整倍体检出率２９．８％，４例完全缓解的患者仍检出ＤＮＡ非整倍体细胞，随化疗进展而消失；对２１例ＡＬ患者骨髓细胞Ｓ％与化疗后原始细胞下降指数（ＢＲＩ），缓解率和复发的关系进行探讨，发现高Ｓ期组化疗后ＢＲＩ明显高于低Ｓ期组（Ｐ＜０．０１），但高Ｓ期组获得缓解的患者易于复发，而缓解率两组之间无差异（Ｐ＞０．０５）．Objective: To investigate the marrow cell circle distribution, aneuploid, and its relation with therapeutic effects in adult acute leukemia (AL) patients. Method: Bone marrow cellular DNA content was assessed in 57 patients with acute leukemia by flow cytometry, the relationship between S phase percent and response to antileukemic effect of induction chemotherapy was evaluated. Results: Forty six untreated patients had a smaller S phase compartment than patients with complete remission and normal control. Acute promyelocytic leukemia (M 3) had the smallest S phase compartment. The proportion of patients with DNA aneuploidy in acute leukemia was 29.8%. Abnormal DNA content cells in four patients after complete remission were still determined. The relationship between S phase compartment and blast reduction index (BRI) showed that high S phase group was more sensitive than low S phase group in response to chemotherapy, but complete remission patients with high S phase compartment were apt to relapse. Complete remission rate of both groups was of no difference. Conclusion: Analysis of marrow cell DNA in AL patients by FCM can provide the crucial reference data for treatment.

关 键 词：流式细胞仪 急性 白血病 DNA 

分 类 号：R733.710.2[医药卫生—肿瘤]