凋亡基因bcl-xl在骨肉瘤中的表达  

Expression of Apoptosis Gene bcl-xl in Osteosarcoma

作　　者：孟祥龙[1] 王庆一[1] 曲铁兵[1] 王进军[1] 范广宇[2] 王玉峰[2] 

机构地区：[1]首都医科大学附属北京朝阳医院骨科 [2]中国医科大学附属第一临床学院骨科

出　　处：《首都医科大学学报》2005年第6期747-748,共2页Journal of Capital Medical University

摘　　要：目的探讨肿瘤凋亡基因bcl-xl在骨肉瘤发生中的作用。方法采用链霉菌抗生物素蛋白-过氧化酶连接法(SP法)检测27例传统骨肉瘤和22例良性软骨骨瘤中的bcl-xl的表达,表达程度采用半定量方式判定。结果bcl-xl在骨肉瘤中表达的阳性率为100%,软骨骨瘤中表达的阳性率为4.5%,两者比较差异有统计学意义(P<0.01)。结论骨肉瘤不同分化与阳性半定量间无相关性(P>0.05)。bcl-xl蛋白表达与骨肉瘤的发生有关,但与骨肉瘤的分化程度无关。Objective The aim was to study the role of apoptosis gene bcl-xl in the tumorigenesis of osteosarcoma. Methods The expression of bcl-xl was observed by immunohistocbemical assay in twenty-seven classical ostecsarcoma and twenty-two benign bone tumors. The extent of expression was evaluated by using half-quantitative method. Results The positive rate of bcl-xl expression was 100 % in osteosarcoma, 4.5 % in benign osteochondroma. There was a difference with statistical significance （P 〈 0.01） between two positive rates. Conclusion No correlation exists between different differentiation and positive grade （P 〉0. 05）. The expression of bcl-xl is involved in the tumorigenesis of osteosarcoma, but not associated with the differentiation of osteosarcoma.

关 键 词：骨肉瘤 BCL-XL 免疫组织化学 凋亡基因 

分 类 号：R738.1[医药卫生—肿瘤]