MAGE-A1在人脑胶质瘤中的表达及其意义  

Expression of MAGE-Al in Human Glioma and Its Clinical Significance

作　　者：程光[1] 章翔[1] 张赟[2] 陈广生[3] 曹卫东[1] 高大宽[1] 宋蕾[1] 

机构地区：[1]第四军医大学西京医院神经外科,陕西西安710032 [2]第四军医大学免疫教研室,陕西西安710032 [3]第四军医大学病理教研室,陕西西安710032

出　　处：《标记免疫分析与临床》2005年第4期223-225,222,T0001,共5页Labeled Immunoassays and Clinical Medicine

基　　金：国家自然科学基金资助项目:(NO.39970752)

摘　　要：为探讨黑色素瘤抗原A1(melanoma antigen A1,MAGE-A1)蛋白在人脑胶质瘤中的表达与其恶性程度的 关系,用免疫组织化学SABC法检测48例人脑胶质瘤标本、2株人脑胶质瘤细胞系U87、SHG-44及6名正常人 脑组织中MAGE-A1蛋白的表达。同时采用免疫荧光染色后流式细胞仪分析及荧光显微镜观察2株胶质瘤细 胞MAGE-A1的表达。结果显示,MAGE-A1蛋白在Ⅰ-Ⅳ级胶质瘤组织中阳性率分别为25．0％,29．4％, 38．5％和50．0％,正常脑组织无表达。胶质瘤细胞系U87、SHG-44细胞MAGE-A1的表达率分别为77．3％, 95．8％。表明MAGE-A1蛋白表达于人脑胶质瘤细胞,并与其恶性程度相关。To explore the relationship between the expression of melanoma antigen A1 （MAGE- A1） and the malignant level of human glioma, the expression of MAGE- A1 was examined in 48 tumor tissue samples of glioma patients, 2 human glioma cell lines and 6 normal human brain tissue samples by immunohistochemistry. Flow cytometry analysis and fluorescence microscopy were employed to detect the expression of MAGE-A1 in two glioma cell lines U87 and SHG-44 after indirect fluorescent staining. The results showed that the percentage of MAGE-A1 expression in glioma samples with malignancy of grades Ⅰ～Ⅳ was 25.0%,29.4%,38.5% and 50.0%, respectively, whereas there was no expression of MAGE-A1 in normal human brain tissues. Furthermore, the expression rates of MAGE-A1 in U87 and SHG-44 cell lines were 77.3% and 95.8%, respectively. These data suggest that MAGE-A1 is expressed by human gliomas cells and is correlated with the malignancy grade of the glioma.

关 键 词：黑色素瘤抗原A1 胶质瘤 表达 

分 类 号：R739.4[医药卫生—肿瘤]