南京血吸虫扫描电镜观察  

Scanning electron microscopy of Schistosoma nanjingi

作　　者：徐国余[1] 李解权[1] 濮祖茂[2] 杨佩才[1] 

机构地区：[1]江苏省南京市疾病预防控制中心,南京210003 [2]中国药科大学电镜室

出　　处：《中国血吸虫病防治杂志》2005年第6期449-450,T0001-T0002,共4页Chinese Journal of Schistosomiasis Control

摘　　要：目的用扫描电镜观察南京血吸虫体表形态和结构。方法用南京血吸虫的阳性钉螺逸出的尾蚴感染兔,每兔1 200条,感染45-80 d后解剖,获得的血吸虫用4％戊二醛固定。然后按扫描电镜观察要求,清洗、固定、脱水、干燥、镀金,用SX-40扫描电镜进行观察。结果大雄虫背中部体表有粗大的嵴和深的凹陷,腹中部有小棘。小雄虫整个背部和腹部均有小棘。大雌虫体表均有小棘。小雌虫背部和腹面均有小棘,但形状不同,旋转的尾部在同一平面显示出2种体棘;腹吸盘可见轮辐状。雌雄虫体表均可见有纤毛和没有纤毛的感觉乳突。结论南京血吸虫体表存在着与日本血吸虫不同的形态和结构。考虑为一新种血吸虫。Objective To examine the tegmental structure of Schistosoma nanjingi using scanning electron microscopy. Methods Adult schistosomes were obtained from infected rabbits with cercariae shedding from Oncomelania snails, which were infected with miracidia of Schistosoma nanjingi. The adult schistosomes were fixed with 4% glutaradehyde, and then, the samples were prepared with the conventional procedures and the schistosomes were examined with a scanning electron microscope （SX-40）. Results There were two types of male and female adult worms. For the big male worm, there were big spines and deep cavity on the surface of middle back and some small spines on the surface of middle abdomen; for the small male worm, there were many small spines on the surface of whole back and abdomen. As the big female worm, there were some small spines on the whole tegumental surface. As the small female worm, there were some small spines on the surface of back and abdomen, but the shape of spines was different between the spines of back and abdomen. On the tail surface of the small female worm, there were two types of spines. The spokelike acetabulum was found. The sensory organelle papillae with or without cilia were found on the tegmental surface of both male and female woems. Conclusion The tegmental structure of Schistosoma nanjingi is much different from that of Schistosoma japonicum.

关 键 词：扫描电镜 南京血吸虫 日本血吸虫 

分 类 号：R383.24[医药卫生—医学寄生虫学]