NaOH皂化反相高效液相色谱法测定雨生红球藻中虾青素  

Determination of Astaxanthin in Haematococcus Pluvialis by HPLC after Saponification with NaOH

作　　者：齐安翔[1] 蔡明刚[1] 张英[1] 金贵娥[1] 

机构地区：[1]厦门大学海洋系

出　　处：《分析科学学报》2005年第6期619-622,共4页Journal of Analytical Science

基　　金：福建省科技项目(No.2002Y052);厦门市科技项目(No.3052Z20031086);厦门大学"挑战杯"科研计划

摘　　要：建立了一种雨生红球藻中虾青素酯皂化的反相高效液相色谱方法.采用二极管阵列检测器(DAD)在476 nm处进行检测,虾青素在15 min内得到了较好的分离.虾青素的含量在1～15 mg/L范围内与峰面积具有良好的线性关系,相关系数为0.999 3;方法的检出限为0.049 mg/L,RSD为3.27%,及回收率为100.7%.应用本法测定出雨生红球藻中虾青素的总含量为9.79～24.70 mg/L.A reversed-phase high performance liquid chromatographic （RP-HPLC） method was developed for the separation of astaxanthin ester that have been saponified from Haematococcus pluvialis. Chromatographic separation was performed on Hypersil ODS column （5μm, 4.6 × 250 mm, Germany） at room temperature with an eluent of the mixlure of acetonitrile and methanol （75：25,V/V） al a flow rate of 1. 0 mL/min. The eluate was monitored by a diode array detector at 476 nm. Separation and identification of astaxanthin was completed within 15 min. The detection limit and the linear range of astaxanthin were 0. 049 mg/L and 1～15 mg/L （r=0. 9993）,respectively. The pre （RSD） was 3.27%, and average recovery was 100.7%. The method was rapid, accurate. cision of the method simple, precise and

关 键 词：虾青素 雨生红球藻 虾青素酯 皂化 高效液相色谱法 

分 类 号：O657.72[理学—分析化学] Q949.29[理学—化学]