非水体系毛细管电泳-紫外检测法测定对乙酰氨基苯酚的研究  

Study of Parecetamol Determination by Capillary Electrophoresis with UV Detection

作　　者：李利军[1] 黄文艺[2] 程昊[2] 孔红星[1] 吴健玲[2] 

机构地区：[1]广西工学院生物与化学工程系,广西柳州545006 [2]广西大学化学与化学工程学院,广西南宁530005

出　　处：《分析科学学报》2005年第6期658-660,共3页Journal of Analytical Science

基　　金：广西教育厅科研基金(No.2000392);广西自然科学基金(No.0481019)

摘　　要：本文报道了用非水相体系高效毛细管电泳-紫外检测法测定对乙酰氨基苯酚的新方法;考察了运行电压、非水相介质和电解质等因素的影响.在25℃下,以50mmol/L NaOH的乙醇溶液为电泳缓冲介质,30 mmol/L乙酸钠的乙醇溶液为样品溶剂;选择重力进样30 s,运行电压一25 kV,及检测波长250 nm.测定对乙酰氨基苯酚的线性范围为5.2～52 μg/mL;检出限为2.88μg/mL;RSD为2.34%.本法应用于维C银翘片中对乙酰氨基苯酚的测定,结果满意.A new method of parecetamol was established by non-aqueous capillary electrophoresis with UV detection. The effects of non-aqueous buffer solution, non-aqueous sample solution, concentration of electrolyte and separation voltage were investigated. An alcohol based buffer solution containing 50 mmol/L sodium hydroxide was used. The used voltage was -25.0 kV. The analytes were detected at 250 nm. Linear calibration was obtained in the range 5. 2 ～52μg· mL^-1 for parecetamol, and the correlation coefficient was 0. 998. The detection limit was 2. 88μg·mL^-1. The recoveries of parecetamol ranged from 97. 2% to 101.7%.

关 键 词：对乙酰氨基苯酚 维C银翘片 非水相体系 毛细管电泳 紫外检测 

分 类 号：O657.8[理学—分析化学] R971.1[理学—化学]