脂质体介导nm23-h1质粒体内转染腺样囊性癌可行性  

Feasibility of Cationic Lipid Mediated nm23-h1 Plasmid Transfecting Adnoid Cystic Carcinoma in vivo

作　　者：李文[1] 温玉明[2] 彭文珍[3] 

机构地区：[1]四川大学华西医院耳鼻喉科 [2]四川大学华西口腔医学院口腔颌面外科学教研室 [3]四川大学华西基础医学与法医学院分子生物学实验室,四川成都610041

出　　处：《华西口腔医学杂志》2005年第6期471-473,共3页West China Journal of Stomatology

基　　金：国家自然科学基金资助项目(39870746)

摘　　要：目的研究阳离子脂质体介导nm23_h1质粒体内转染腺样囊性癌的可行性。方法将腺样囊性癌细胞株体外培养后移植入40只裸鼠体内。ACC瘤体长至直径1 cm左右时,用脂质体包裹nm23_h1质粒进行瘤体内注射,每次注入质粒-脂质体复合物0.1 ml,其中10只裸鼠每只注射2次,10只裸鼠注射1次,10只作空白对照,10只裸鼠多针道注射0.2 ml质粒及脂质体复合物。注射后2、3、7 d处死动物,获取肿瘤标本作常规组织学及免疫组化研究。结果瘤体内注射质粒-脂质体复合物3 d后肿瘤细胞内均有nm23_h1表达,7 d后表达增加,肿瘤细胞外间质增加,2次注射较1次注射、多针道注射较单一针道注射有更多的细胞呈阳性表达。结论脂质体介导nm23_h1质粒体内转染腺样囊性癌可获得小范围阳性表达,但还不具备临床实施的可能性。Objective To investigate the feasibility of plasmid nm23-h1 transfection on high metastatic potential adnoid cystic carcinoma（ACC-M） cell hne mediated by cationic hpid. Methods ACC-M cell were implanted in the maxillofacial region in each of dO BALB/c nude mice. After the tumor growth to 1 cm in diameter,0.1 ml Lipofctamine-nm23-h1 plasmid complex were injected intratumorally in 10 mice, 3 days after the first injection, 10 mices injected for twice, 10 mice as plamid-blank control, another 10 mice were injected 0.2 ml complex, 2,3,7days after the injection, the mice were killed and the specimen for HE and immunohistological chemistry study. Results nm23-h1 expression initiated in the tumor cells 3 days after the complex injection, 7 days later, the expression level increased accompanying with extracellular matrix increase, twice injection and multiple channel injection would gain better nm23-h1 expression than once injection and single-channel injection respectively. Conclusion Cationic hpid mediated nm23-h1 plamid transfecting adnoid cystic carcinoma can gain small range positive expression, but the results give little prospect for further clinical treatment in such a manner.

关 键 词：基因治疗 NM23 阳离子脂质体 腺样囊性癌 

分 类 号：R73-3[医药卫生—肿瘤]