永生化成牙本质细胞表达牙本质基质蛋白的实验研究  

Immortalized Human Odontoblast-like Cell Line Expressed Dentin Extracellular Matrix in vitro

作　　者：王捍国 肖明振 赵守亮 郝建军 张旻 

机构地区：[1]第四军医大学口腔医院牙体牙髓病科,陕西西安710032

出　　处：《华西口腔医学杂志》2005年第6期518-521,共4页West China Journal of Stomatology

基　　金：国家自然科学基金资助项目(39970792;30270374);教育部回国人员启动基金资助项目(2000HG003);第四军医大学学术新人资助基金资助项目(2003年)

摘　　要：目的探讨永生化人成牙本质细胞样细胞系hTERT_hOd_l表达牙本质基质蛋白的情况。方法矿化液培养hTERT_hOd_l细胞5周,检测骨钙素(OC)分泌量和碱性磷酸酶(ALP)活性。采用免疫组织化学、RT_PCR和原位杂交方法检测Ⅰ型胶原、骨涎蛋白(BSP)、牙本质基质蛋白1(DMP1)以及成牙本质细胞标志物牙本质涎磷蛋白(DSPP)和牙本质涎蛋白(DSP)在细胞中的表达。结果在矿化液诱导下,hTERT_hOd_l细胞ALP活性和OC分泌量升高。hTERT_hOd_l细胞在mRNA水平上表达BSP、DMP1和DSPP,在蛋白质水平上表达DSP和Ⅰ型胶原。结论hTERT_hOd_l细胞在体外表达牙本质基质蛋白,具有矿化的潜能。Objective To determine whetiler dentin matrix proteins were expressed by the human odontoblast-like cell line hTERT-hOd-1 in vitro. Methods Collagen type Ⅰ , bone sialoprotein （BSP）, dentin matrix protein 1 （ DMP1 ） and the marker for odontoblast, dentin sialophosphoprotein （DSPP） and dentin sialoprotein（DSP） were detected in these cells by immunohistochemistry, RT-PCR and in situ hybridization. During being cultured in mineralizing medium for 5 weeks, the secretion of OC and activity of ALP were measured once a week. Results DSPP, DMP1, BSP and collagen type I were expressed in hTERT-hOd-1 either at mRNA or protein level. Under the induction of mineralizing medium, the cells showed higher activity of ALP and increased secretion of OC. Conclusion hTERT-hOd-1 expressed dentin extracellular matrix in vitro, which means the cell line has the potential of mineralization.

关 键 词：成牙本质细胞 牙本质基质蛋白 矿化 

分 类 号：R78[医药卫生—口腔医学]