α-乙酰乳酸脱羧酶在大肠杆菌中的克隆表达  

Cloning and Expression of α-Acetolactate Decarboxylase Gene in E.coli

作　　者：李联正[1] 张惟材[1] 汪建华[1] 何维明[2] 

机构地区：[1]军事医学科学院生物工程研究所,北京100071 [2]西北农林科技大学动物科技学院,陕西杨凌712100

出　　处：《生物技术通讯》2005年第6期618-620,共3页Letters in Biotechnology

摘　　要：根据已知的α-乙酰乳酸脱羧酶(ALDC)的基因序列,通过PCR获得了枯草芽孢杆菌168的ALDC基因,将该基因克隆到克隆载体pUC18和表达载体pQE60中,构建了pUC18-ALDC和pQE60-ALDC,经DNA测序证明序列正确。重组大肠杆菌DH5α/pQE60-ALDC经IPTG诱导能够高表达ALDC。对该酶进行了活力测定,结果显示工程菌产酶活力为0.11U/mL。α-acetolactate decarboxylase（ALDC） was amplified from Bacillus subtilis 168 by PCR. The ALDC gene was cloned in plasmid pUC18 and pQE60, recombinant plasmid pUC18-ALDC and pQE60-ALDC were obtained. The amplifed fragment was confirmed to be an ALDC gene by DNA sequencing. Under T5 promoter, recombinant ALDC was expressed by E.coli DH5a/pQE60-ALDC in high level induced with IPTG and assaied the activity of ALDC. The biological activity of the recombinant ALDC is determined to be 0.11 U/mL.

关 键 词：Α-乙酰乳酸脱羧酶 克隆 表达 大肠杆菌 

分 类 号：Q785[生物学—分子生物学] Q786