热休克蛋白在宫颈病变中的表达(英文)  

Expressions of heat shock protein in uterine cervix with different pathological changes

作　　者：鲍嫘 赵恩锋 梁龙 李冬萍 

机构地区：[1]Department of Clinical Medicine, Graduate School of Zhejiang University, Hangzhou, Zhejiang 310031 [2]Department of Gynecology and Obstetrics, General Hospital of PLA, Beijing 100853 [3]Institute of Bio-engineering, Academy of Military Medical Sciences, Beijing 100071

出　　处：《解放军医学杂志》2005年第12期1043-1045,共3页Medical Journal of Chinese People's Liberation Army

摘　　要：目的研究几种主要热休克蛋白(heat shockprotein,HSP)在不同病理改变的宫颈组织中的表达。方法根据病理诊断,把478例宫颈活检标本分为宫颈癌组(63例)、宫颈上皮内瘤形成组(CIN组,106例)、宫颈炎组(293例)及正常宫颈组(16例)。采用以特异性复合cRNA为内参照的定量RTPCR法,检测各组标本的HSP70、HSP90α和HSP90βmRNA的表达。结果(1)HSP70、HSP90α和HSP90βmRNA在宫颈浸润癌、CIN、宫颈炎及正常宫颈组织中的表达量均呈现阶梯式下降,差异均有极显著意义(P<0.01)。(2)HSP90βmRNA在晚期宫颈癌中的表达比早期表达高(P<0.05);HSP70和HSP90βmRNA在低分化宫颈癌中的表达比高分化表达高(P<0.05);而HSP70、HSP90α和HSP90βmRNA在不同组织类型宫颈癌中的表达均无显著性差异(P>0.05)。结论热休克蛋白在宫颈癌的发生和发展中起着重要的协同作用。HSP70和HSP90α与宫颈癌细胞的转化和增殖密切相关,HSP90β可能参与细胞的分化。Objective To study the expressions of main subtypes of heat shock protein （HSP） in uterine cervix with different pathological changes. Method 478 cases of cervical biopsy specimen were divided into invasive carcinoma of cervix group （63 cases）, cervical intraepithelial neoplasia group （CIN, 106 cases）, chronic cervicitis group （293 cases） and normal uterine cervix （16 cases） according to pathological diagnosis. The expression levels of HSP70, HSP90α and HSP90β mRNA were detected by quantitative RT-PCR with specific complex cRNA as internal control. Results （1） The expressions of HSP70, HSP90α and HSP90β mRNA were significantly downtrend stepwise in invasive carcinoma of cervix, CIN, chronic cervicitis and normal cervix tissue. （P＜0.01, respectively）. （2）In the invasive carcinoma of cervix group, the expression level of HSP90β mRNA was higher in advanced stage （FIGOⅡb-Ⅳ） compared with incipient （FIGOⅠa-Ⅱa） cancer of the cervix. （P＜0.05）. （3）The expressions of HSP70 and HSP90β mRNA were each higher in poorly differentiated tumor than in well-differentiated tumor. （P＜0.05, respectively）. （4）The expression levels of all three HSP mRNA had no significant differences were observed with different histological types of cervical cancer. （P＞0.05）. Conclusions Heat shock protein may play some important roles in malignant transformation of cervix cell and aggravation of cervix cancer. HSP70 and HSP90α may promote cancer cell transition and proliferation, and HSP90β may participate in cell differentiation.

关 键 词：宫颈疾病 病理学 临床 热休克蛋白质类 

分 类 号：R737.33[医药卫生—肿瘤]