反义hTERT对胃癌细胞SGC7901 VEGF及其受体表达的影响  

The influence of anti-sense hTERT on the expression of VEGF and Flt in SGC7901 cells

作　　者：司艺玲[1] 韩为东[1] 赵亚力[1] 宋海静[1] 李琦[1] 郝好杰[1] 

机构地区：[1]解放军总医院基础医学研究所分子生物学研究室,北京100853

出　　处：《解放军医学杂志》2005年第12期1061-1063,共3页Medical Journal of Chinese People's Liberation Army

基　　金：解放军总医院"十五"青年基金资助课题(编号01YQ20)

摘　　要：目的研究反义hTERT对胃癌细胞SGC7901VEGF及其受体表达的影响,探讨其对肿瘤发展和转移的影响。方法以端粒酶逆转录酶组分hTERTcDNA为模板构建反义hTERT逆转录病毒表达载体,包装重组逆转录病毒后感染胃癌SGC7901细胞,用半定量RTPCR检测基因转染前后VEGFC及其受体Flt4mRNA表达水平的变化,免疫组化法检测VEGFC、Flt4蛋白表达。结果反义hTERT稳定转染后SGC7901细胞VEGFC及其受体VEGFR3(Flt4)mRNA和蛋白表达均受到明显抑制。结论反义hTERT可有效抑制胃癌细胞VEGFC及其受体Flt4的表达,阻断肿瘤血管和淋巴途径转移。Objective To investigate the effects of anti sense hTERT on the expression of VEGF and the receptor in SGC7901 cells. and study the influence in angiogenesis and progression in gastric carcinoma. Methods SGC7901 cell line was transfected by the recombinant virus containing sense and anti-sense hTERT cDNA. Then the expression of VEGF-C and Fit 4 was observed with RT-PCR, distribution of cell cycles was determined with flow cytometry. The expression of VEGFC and Fit 4 protein was assessed with immunohisto- chemistry. Result The distribution of cell cycle of antisense hTERT transfected SGC7901 cells was changed, and the mRNA and protein expression of VEGFC and Flt 4 was significantly down regulated. Conclusion Anti sense hTERT can act as an agent for inhibiting VEGF-C and Flt-4 mRNA and protein level, and it blocks tumor angiogenesis and lympbogenous metastasis.

关 键 词：DNA 反义 人端粒酶逆转录酶 胃肿瘤 血管内皮生长因子类 

分 类 号：R734[医药卫生—肿瘤]