胞浆CD79a和其它B系相关抗原在急性白血病细胞表达的比较研究(英文)  

Comparative Study of Expressions of Cytoplasmic CD79a and Other B-Lymphoid Immunomarkers in Acute Leukemic Cells

作　　者：张敬宇[1] 吕涛 杨敬慈[1] 潘崚[1] 罗建民[1] 杨琳[1] 姚丽[1] 董作仁[1] 徐世荣[1] 

机构地区：[1]河北医科大学第二医院血液科,石家庄050000 [2]河北省消化病研究所,石家庄050000

出　　处：《中国实验血液学杂志》2005年第6期954-958,共5页Journal of Experimental Hematology

摘　　要：为了比较胞浆CD79a(CyCD79a)与包括胞浆CD22(CyCD22)、CD19、CD20和CD10等其它4种常用B系相关抗原在各种急性白血病细胞上的表达情况并寻找针对急性前体B淋巴细胞白血病(pBALL)的最敏感和特异的免疫学标志,对采用流式细胞术方法检测的221例成人和儿童急性白血病的免疫表型资料及常规细胞形态学和酶细胞化学染色资料进行了回顾性分析。另外,还对其中的45例急性早幼粒细胞白血病和13例怀疑存在AML1/ETO和CBFβ/MYH11等融合基因的急性髓细胞白血病(AML)进行了细胞遗传学和/或分子生物学检测。结果发现,对于pBALL来说,在这5种常用的B系抗原中,CyCD79a的敏感性最高,而CyCD22的特异性最好。CyCD79a在全部58例pBALL中的表达率为100%,而CyCD22在全部147例AML和15例急性前体T淋巴细胞白血病(pTALL)中则无1例表达,即在非pBALL的急性白血病中的表达率为0%。结论:联合检测CyCD79a和CyCD22是诊断pBALL的最合适的免疫学指标,能够准确地将pBALL与AML和pTALL相鉴别。To evaluate the expression of cytoplasmic CD79a （CyCD79a） and other commonly used B-lymphoid immunomarkers including cytoplasmic CD22 （CyCD22）, CD19, CD20 and CD10 in various acute leukemia cells and to define the most sensitive and specific markers in the diagnosis of precursor B-cell acute lymphoblastic leukemia （ pB-ALL）, the immunophenotypic data from 221 de novo adult and pediatric acute leukemia patients as studied using multiparameter flow cytometry in addition to routine morphologic and enzyme cytochemical assay, were retrospectively analyzed. Cytogenetic and/or molecular biological data in all 45 cases of acute promyelocytic leukemia （APL） and 13 cases of acute leukemia suspected as AML with the fusion genes such as AML1/ETO and CBFβ/MYH11 were investigated.The results showed that CyCD79a and CyCD22 were the most sensitive and specific markers respectively for pB-ALL.Expression of CyCD79a was seen in 100% of 58 cases of pB-ALL. At the same time, none （0%） of all 147 cases of acute myeloid leukemia （AML） and 15 cases of precursor T-cell acute leukemia （pT-ALL） was positive for CyCD22.The conclusion is made that united detection of CyCD79a and CyCD22 is the optimal immune combination for the diagnosis pB-ALL and the distinguishing pB-ALL with AML and pT-ALL.

关 键 词：胞浆CD79a 胞浆CD22 CD19 CD20 CD10 急性白血病 

分 类 号：R733.71[医药卫生—肿瘤] R733.1[医药卫生—临床医学]