GSK3在人、鼠、猪的肺组织及培养的猪支气管上皮细胞中的表达  

EXPRESSION OF GLYCOGEN SYNTHASE KINASE 3 IN LUNG TISSUES OF HUMAN,RAT,MOUSE,PIG AND CULTURED PIG BRONCHIAL EPITHELIAL CELLS

作　　者：田丹[1] 王曦[1] 陈文书[1] 吴人亮[1] 

机构地区：[1]华中科技大学同济医学院病理学系卫生部呼吸系疾病重点实验室,武汉430030

出　　处：《中国组织化学与细胞化学杂志》2005年第6期598-602,共5页Chinese Journal of Histochemistry and Cytochemistry

基　　金：国家自然科学基金资助项目(30200115和30470757)

摘　　要：目的探讨GSK3在动物肺组织及培养的猪支气管上皮细胞中的表达。方法用免疫组化,免疫细胞荧光法检测GSK3在人、大鼠、小鼠和猪的肺组织及培养的猪支气管上皮细胞中的表达。结果GSK3α和β广泛表达于人、鼠和猪的肺组织中,定位于胞浆。它们在几种动物肺组织中的表达分布大致相似,主要见于各级支气管上皮细胞、肺泡上皮细胞、粘膜平滑肌细胞和粘膜下腺体。但GSK3α在软骨细胞中表达明显强于GSK3β;几种哺乳动物肺组织中均未检测到GSK3的磷酸化。免疫荧光检测培养的猪支气管上皮细胞中有丰富的GSK3α、β的表达,磷酸化的GSK3信号弱。结论GSK3α、β丰富表达于不同种属哺乳动物肺组织的支气管上皮、腺体以及肺泡上皮,提示其可能在肺组织的生理功能和病理发生机制中起着重要作用。Objective To investigate the expression of glycogen synthase kinase 3 （GSK3） in the lung tissues of human, rat, mouse and pig and in cultured pig bronchial epithelial cells. Methods The expression of GSK3 in lung tissue or cultured bronchial epithelial cells was observed by immunohistochemistry or immunofluorescence respectively. Results Immunostaining showed that both GSK3a and GSK3β were prominently expressed in the plasm of bronchial epithelial cells, submucosal gland cells, smooth muscle cells and alveolar epithelial cells of the above mammals. However, there were unexpected high levels of GSK3α in cartilage lacuna cells when compared with those of GSK3β. No signals of phosphorylated GSK3a/β were observed in pig the lung tissues . Immunofluorescence demonstrated that the expressions of GSK3a and GSK3β were high but phosphorylated GSK3a/β was low in pig cultured bronchial epithelial cells. Conclusion The high and extensive expression of GSK3 in the major cells of the lung tissues of investigates mammals indicates that it may play an important role in the physiological and pathological processes of the lung.

关 键 词：GSK3 肺组织 猪支气管上皮细胞 

分 类 号：R322.3[医药卫生—人体解剖和组织胚胎学]