一氧化氮供体型齐墩果酸衍生物ZCⅡ_2对早期实验性肝纤维化的保护作用  

Studies on anti-early hepatic fibrosis of no donating-oleanlic acid derivatives in rats

作　　者：陈秀英[1] 季晖[1] 张奕华[2] 陈莉[2] 

机构地区：[1]中国药科大学药学院药理学教研室 [2]中国药科大学新药研究中心

出　　处：《中国临床药理学与治疗学》2005年第11期1261-1265,共5页Chinese Journal of Clinical Pharmacology and Therapeutics

摘　　要：目的:观察一氧化氮供体型齐墩果酸衍生物ZCⅡ2对早期实验性肝纤维化的保护作用。方法:用CCl4诱导大鼠肝纤维化模型,ZCⅡ2以128mg·kg-1和64mg·kg-1的剂量给大鼠连续灌胃给药30d,测定血清总蛋白(TP)、白蛋白(ALB)、谷丙转氨酶(ALT)、谷草转氨酶(AST)、一氧化氮(NO)以及血清透明质酸(HA)、层粘蛋白(LN)和Ⅲ型前胶原(PCⅢ)的含量,计算白蛋白与球蛋白的比例(白球比例);测定肝组织中丙二醛(MDA)和谷胱甘肽过氧化物酶(GSHPx)及一氧化氮合酶(NOS)的含量,并行肝组织病理组织学检查。结果:与模型组相比,ZCⅡ2高剂量能显著降低谷丙转氨酶、谷草转氨酶含量,增加白蛋白含量,提高白球比例,显著降低血清透明质酸、层粘蛋白和Ⅲ型前胶原含量,明显改善肝组织病理损伤。结论:一氧化氮供体型齐墩果酸衍生物ZCⅡ2对大鼠早期实验性肝纤维化具有一定的保护作用。AIM： To investigate protect effect of the NO donating-oleanlic acid derivatives on hepatic fibrosis in rats. METHODS： The rat model with early hepatic fibrosis was induced by carbon tetrachloride （CC14）. ZC Ⅱ 2 at the dose of 128 and 64 mg·kg^-1 had been given orally for 30 days. Serum level of the total protein （TP）, albumin （ALB）, the albumin/globulin （A/G） and ALT, AST. The hyaluronic acid （HA）, laminin （LN）, the procollagen type Ⅲ （PCⅢ） and the level of MDA, GSHPx in liver tissues were detemained. Pathological examination to reveal the extent of liver damages was observed. RESULTS ： ZC Ⅱ 2 at the dose of 128 mg·kg^- 1 increased the serum TP, ALB, and A/G and decreased HA, LN, PC IH, ALT, and AST more significantly than the model group, and hepatic pathological injury was abated to some degree. CONCLUSIONS： ZCⅡ2 at the dose of 128mg·kg^-1 can attenuate liver damages, protect against lipoperoxidation and attenuate hepatic fibrosis induced by CCl4.

关 键 词：一氧化氮 齐墩果酸 肝纤维化 一氧化氮供体 NCX-1000 

分 类 号：R965.1[医药卫生—药理学]