塞来昔布对胃癌细胞生长及ERK2表达的影响  

Effects of celecoxib on cellproliferation and ERK2 expression of ERK2 in gastric cancer cells

作　　者：张勇[1] 蒋明德[1] 曾维政[1] 徐辉[1] 熊碧君[1] 翁敏[1] 

机构地区：[1]中国人民解放军成都军区总医院消化内科,四川省成都市610083

出　　处：《世界华人消化杂志》2005年第18期2213-2216,共4页World Chinese Journal of Digestology

摘　　要：目的:观察选择性环氧合酶-2抑制剂塞来昔布对胃癌细胞株SGC7901增殖的影响,及细胞增殖与细胞外信号调节激酶2(ERK2)表达的关系.方法:MTT法检测塞来昔布对胃癌细胞生长的影响;TUNEL法及流式细胞仪检测细胞凋亡率;免疫组化(SP法)检测细胞磷酸化ERK2表达,图像分析系统计量.结果:塞来昔布呈剂量依赖方式抑制胃癌细胞的生长,诱导细胞凋亡,抑制胃癌细胞磷酸化ERK2表达.塞来昔布10,20,40,80,160μmol/L作用48h后其胃癌细胞生长抑制率分别为9.8%,30%,58.9%,76.3%,88.3%;TUNEL染色显示胃癌细胞具有典型的凋亡细胞形态学特征;流式细胞仪显示胃癌细胞凋亡率(4.23±0.81%,15.5±2.1%,24.35±2.32%,31.52±3.64%,45.82±5.92%)显著高于对照组(1.85±0.31%,P<0.01);其磷酸化ERK2平均吸光度(2.96±0.24,P>0.05;2.56±0.24,P<0.05;2.04±0.20,P<0.01;1.68±0.16,P<0.01;1.52±0.09,P<0.01)显著低于对照组(3.32±0.28).结论:塞来昔布抑制胃癌细胞增殖并诱导细胞凋亡,这可能与其抑制ERK2信号传导通路有关.AIM： To explore the role of celecoxib, a selective COX-2 inhibitor, in the proliferation of human gastric carcinoma cells SGC7901, and to investigate the relationship between cell proliferation and the expression of extracellular signal-regulated kinase 2 （ERK2）, METHODS： The cell proliferation of SGC7901 cells was measured by MTT assay, and the apoptoticsis rate of the cells was examined by TUNEL staining method and flow cytometry, The expression of phosphated ERK2 was detected by immunohistochemistry and quantified measured by image analysis systems, RESULTS： Celecoxib inhibited the proliferation of SGC7901 gastric carcinoma cells and the expression of phosphated ERK2, as well as induced the apoptosis of the SGC-7901 cells in a dose-dependent manner. The growth inhibitory rates of SGC-7901 cells treated with 10, 20, 40, 80 and, 160 μmol/L celecoxib for 48 h were 9.8%, 30%, 58.9%, 76.3%, and 88.3%, respectively. The SGC7901 cells presented typical morphological features of apoptosis by TUNEL staining. By flow cytometry, the apoptosistic rates of SGC-7901 cells （4.23 ± 0.81%, 15.50 ± 2.10%, 24.35 ± 2.32%, 31.52±3.64%, and 45.82±5.92% for 10, 20, 40, 80 and 160 pmol/L celecoxib, respectively） were significantly higher than those inof the control group（1.85 ± 0.31%, P 〈0.01）. The optical density value of ERK2 of SGC7901 cells （10, 20, 40, 80 and 160 pmol/L celecoxib： 2.96 ± 0.24, P〉0.05; 2.56 ± 0.24, P 〈0.05; 2.04 ± 0.20, P 〈0.01; 1.68 ± 0.16, P〈0.01; 1.52 ± 0.09, P 〈0.01） were significantly lower than that ofin the control group （3.32 ± 0.28）. CONCLUSION： Celecoxib can inhibit the growth and induce the apoptosis of SGC7901 cells, and its mechanism may and induce the cell apoptosis. This effect may relate to the inhibition of ERK2 kinase signaling pathway.

关 键 词：塞来昔布 细胞外信号调节激酶2 胃癌细胞株 SGC790 

分 类 号：R735.2[医药卫生—肿瘤]