大鼠局灶脑缺血后AC133抗原表达的初探  

AC133 expression after focal cerebral ischemia-reperfusion in rat

作　　者：胡湘蜀 周东[2] 罗祖明[2] 

机构地区：[1]广东三九脑科医院神经内科,广州510510 [2]四川大学华西医院神经内科

出　　处：《卒中与神经疾病》2005年第6期350-351,355,共3页Stroke and Nervous Diseases

摘　　要：目的探讨AC133抗原在脑缺血再灌注脑组织中是否表达。方法采用大鼠大脑中动脉缺血再灌注线栓法模型,将成年SD雄性大鼠随机分为(1)正常组,(2)假手术组,(3)动物模型组,分别取缺血再灌注2、3、4、7d作为观察点,每个观察点6只大鼠,共36只大鼠,根据神经功能评分纳入实验,并采用免疫组化染色法检测脑组织AC133抗原表达。结果大脑缺血再灌注后4d AC133蛋白在大脑半球梗死区周围的部分中、小血管内皮细胞胞浆染色阳性,而再灌注后2、3、7d无阳性表达。结论大鼠脑缺血再灌注4d脑梗死边缘区域部分血管内皮细胞AC133抗原表达阳性。AC133抗原可能参与大脑局灶缺血后的血管内皮功能。Objective This study was intended to determine whether AC133 expresses or not after local brain ischemia in rat. Methods Right middle cerebral artery suture occlusion（MCAO） model was used. 36 adult 817） male rats were randomly divided into 3 groups. They were control, sham-control and animal models which included 2,3,4,7 d reperfusion groups. After right middle cerebral artery occluded, the rats were suffered from ischemia for 2 hours, then reperfusion was allowed for different time as mentioned above before decapitation, Cerebral AC133 antigen expression was tested by immunohistochemistry. Result AC133 antigen expression was only observed in certain endothelial cells in areas around infarct core in 4 days. Conclusion AC133 antigen may take part in vessel function in rat MCAO models after focal brain ischemia.

关 键 词：脑局灶缺血再灌注 AC133 大鼠 

分 类 号：R743[医药卫生—神经病学与精神病学]