肢体缺血预适应的小肠保护作用及与一氧化氮／内皮素-1的关系  

Protection of the limb iischemic preconditioning on the injury of gut is concerned with NO/ET-1 system in rats

作　　者：杨全会[1] 张连元[1] 董淑云[1] 门秀丽[1] 王保强[2] 要瑞丽[1] 张一兵[1] 

机构地区：[1]华北煤炭医学院基础部病理生理学教研室,河北唐山063000 [2]华北煤炭医学院附属开滦医院肝胆外科,河北唐山063000

出　　处：《中国病理生理杂志》2005年第12期2378-2381,共4页Chinese Journal of Pathophysiology

摘　　要：目的:观察一氧化氮／内皮素-1(NO/ET-1)失衡与肢体缺血再灌注(L／R)后小肠损伤的关系,以及缺血预适应(IPC)对NO/ET-1系统的调节作用。方法:雄性WISTAR大鼠18只,随机分为对照(CONTROL)组,缺血再灌注 (IR)组和缺血预适应(IPC+IR)组,每组6只,分别测定血浆和小肠组织二氨氧化酶(DAO)、一氧化氮(NO)、内皮素- 1(ET-1)、NO/ET-1比值的含量变化及小肠组织的髓过氧化物酶(MPO)、DNA双链百分率(RATIO OF DNA CHAIN％)、总一氧化氮合酶(TNOS)、诱导型一氧化氮合酶(INOS)、结构型一氧化氮合酶(CNOS)的水平;免疫组化法检测小肠组织的诱导型一氧化氮合酶(INOS)、内皮型一氧化氮含酶(ENOS)的表达。结果:IR组血浆和小肠组织NO、ET-1,血浆DAO 及组织MPO均明最高于对照组,而NO／ET-1的比值,组织DAO及DNA双链百分率明显少于对照组;小肠粘膜INOS 的表达及总NOS活性高于对照组,CNOS(主要为ENOS)的表达少于对照组。IPC+IR组血浆和小肠组织NO、ET-1, 血浆DAO及组织MPO均明显少于IR组,而NO/ET-1的比值,组织DAO及DNA双链百分率却明显高于IR组;小肠粘膜INOS的表达及总NOS活性少于IR组,CNOS(主要为ENOS)的表达高于IR组。结论:肢体I/R后小肠损伤与 NO／ET-1失衡有关,IPC对肢体IR继发的小肠粘膜损伤的拮抗作用可能通过对NO/ET-1系统的调节作用而介导, 此时内皮源的NO产生增加,非内皮源的NO产生减少。AIM： To study the relationship between disturbance of nitric oxide/endothelin - 1 （NO/ET- 1 ） and the injury of gut following limb ischemia - reperfusion （I/R） in rats as well as the regulation of NO/ET - 1 system by limb I/R preconditioning （IPC）. METHODS： A limb ischemia- reperfusion injury model in rats was established. The animals were randomly divided into three groups： control group, IR group and IPC group. The contents of diamide oxidase（DAO）, nitric oxide （NO）, endothelin - 1 （ ET - 1 ） and ratio of nitric oxide/endothelin - 1 （ NO/ET - 1 ） in the plasma and the gut were measured. The leavels of myeloperoxidase, ratio of DNA chain （%）, total nitric oxide synthase （tNOS）, inducible nitric oxide synthase （iNOS） and constitutive nitric oxide synthase （cNOS） in the gut were determined. The expression of iNOS and endothelial NOS （eNOS） were detected by the immunohistochemical methnd. RESULTS： It was found that the levels of NO, ET- 1 in the plasma and the gut tissue all increased after reperfusion, while the values of NO/ET - 1 decreased. The values of DAO in the plasma and MPO in the gut increased, while the contents of DAO and the ratio of DNA chain （ % ） in the gut decreased. The expression of iNOS elevated, cNOS （ mainly eNOS） reduced and total NOS increased. The protection of the limb IPC attenuated the disturbance of NO/EF - 1. CONCLUSION： The intestinal injury following limb I/R is related to the disturbance of NO/ET - 1. The protection of the limb IPC might be conducted by its regulating NO/ET- 1 system. The endothelial NOS increases and non- endothelial NOS decreases in this situation.

关 键 词：再灌注损伤 一氧化氮 内皮缩血管肽1 缺血预处理 

分 类 号：R363[医药卫生—病理学]