GP96-肽复合物免疫小鼠脾淋巴细胞CD8^+细胞内的CA^(2+)浓度变化  

Intracellular Ca^(2+) mobilization in mouse CD8^+ T-lymphocytes immunized with gp96 - peptide complexes

作　　者：张天一[1] 王芳[1] 林琳[1] 顾君一[1] 

机构地区：[1]南通大学神经再生重点实验室,江苏南通226001

出　　处：《中国病理生理杂志》2005年第12期2410-2413,共4页Chinese Journal of Pathophysiology

基　　金：江苏省高校自然科学研究指导性计划项目(NO.03KJD320183)

摘　　要：目的:检测GP96-肽复合物免疫小鼠脾淋巴细胞特定亚群CD8+细胞内的钙离子浓度变化,从而进一步探讨GP96-肽复合物疫苗的免疫效应和机制。方法:以GP96-肽复合物免疫小鼠的脾淋巴细胞作为研究对象, 用QUANTUM-RED标记的抗膜表面抗原CD8+进行膜表面染色的同时,进行FLUO-3/AM负载及细胞内CA2+浓度的检测,以双色流式细胞术检测特定亚群淋巴细胞内的CA2+浓度的变化,钙离子载体A23187、MNCL2和CON A作为小鼠脾淋巴细胞CD8+细胞[CA2+]I的刺激因素。结果:经GP96-肽复合物免疫小鼠脾淋巴细胞CD8+细胞[CA2+]I有一定水平的上调。与对照组相比,A23187和CON A可显著提高GP96-肽复合物免疫小鼠脾淋巴细胞CD8+细胞[CA2+]I 的水平。结论:[CA2+]I浓度的上升可能与GP96-肽复合物促进淋巴细胞CD8+T淋巴细胞的活化及增殖有关。AIM： To further investigate the effect and mechanism of gp96- peptide complexes by observing [ Ca^2 ＋ ]i mobilization in CD8^ ＋ T- lymphoeytes. METHODS： Spleen T- lymphoeytes from the mice immunized with gp96- peptide complexes were used. The cell was stained with quantum - red - labeled anti - CD8 mAb and loaded with Fluo - 3/AM. The doublecolor flow eytometry analysis methed was used to detect [ Ca^2 ＋ ]i changes in CD8 ^＋ T- lymphoeytes from the mice immunized with gp96- peptide complexes. A23187, MnC12 and Con A were. used as stimulators. RESULTS： The [Ca^2＋ ]i in immunized CD8^＋T - lymphoeytes was higher than that in non- immunized cells. Compared with the control group, A23187 and Con A induced higher [Ca^2＋ ]i increases in spleen CD8^＋ T- lymphoeytes of gp96 - peptide complexes - stimulated mice than that in non - immtmized cells. CONCLUSION： CD8 ^＋T- lymphocytes activation is likely due to an elevation of [ Ca^2 ＋ ]i. It suggests that gp96- peptide complexes play a significant role in the modulation of CD8 ^＋ T- lymphoeytes responses.

关 键 词：钙 CD8阳性T淋巴细胞 GP96-肽复合物 

分 类 号：R363[医药卫生—病理学]