银杏叶制剂对脑缺血再灌注后热休克蛋白、C-FOS表达的影响  

Effect of Ginkgo biloba extract on the expression of c-fos and HSP70 following focal cerebral ischemic reperfusion in rats

作　　者：孙琳[1] 刘爱芬[2] 李义召[2] 陈立云[2] 王晓云[1] 韩恩吉[2] 

机构地区：[1]山东大学第二医院神经内科,山东济南250033 [2]山东大学齐鲁医院神经内科,山东济南250033

出　　处：《中国病理生理杂志》2005年第12期2427-2430,共4页Chinese Journal of Pathophysiology

基　　金：山东省科委资助项目(NO.973000056)

摘　　要：目的:研究银杏叶制剂对大鼠脑缺血再灌注后热休克蛋白(HSP)、C-FOS表达的影响,探讨其神经保护机制。方法:采用改良LONGA法复制大鼠局灶脑缺血再灌注模型。56只实验大鼠随机分为正常对照组、假手术组、缺血再灌注组及银杏叶制剂预处理组。银杏组大鼠在实验前灌服银杏制剂2 ML,1日3次,连用5 D。应用HSP70及 C-FOS免疫组化染色、C-FOS MRNA原位杂交、原位细胞凋亡及HE染色等方法观察缺血再灌注不同时点(1 H、6 H、12 H、24 H、3 D、7 D)两者的变化,并对其阳性结果进行半定量分析。结果:银杏制剂预处理组各时段神经细胞缺血程度明显轻于未处理组、TUNEL阳性细胞数明显少于未处理组,HSP70及C-FOS表达的阳性细胞数则明显多于未处理组 (P<0．01)。脑缺血再灌注组1 H时C-FOS即有表达,6 H达高峰,后逐渐下降。再灌注6 H组HSPT0在缺血侧皮质及基底节开始表达,24 H达高峰。再灌注6 H细胞凋亡最显著。结论:银杏制剂可能通过诱导HSP70及C-FOS的表达, 发挥其神经保护作用。AIM： To investigate the influence of Ginkgo biloba extract （GBE） on the expression of c - fos, heat shock protein 70 （HSP70） during focal cerebral ischemic reperfusion in rots. METHODS： The middle cerebral artery occlusion （MCAO） model described by Zea longa was used. Healthy Wistar rots were randomized to 4 groups. Immunohistochemistry, in situ hybridization and terminal deoxynucleotidyl transferase - mediated dUTP nick end labeling （TUNEL） were used to detect the expression of c - fos gene, HSP70 and cell apoptosis at different reperfusion time points： 1, 6, 12, 24 hours and 3, 7 days after recirculation. RESULTS： The positive reactions of both c - los and HSP70 were significantly increased at different reperfusion time in GBE - pretreated ischemia/reperfusion （IR） group than those in ischemia/reperfusion group （ P 〈 0.01 ） and the number of TUNEL - positive cells was reduced in GBE - pretreated IR group. CONCLUSION： The GBE induced the expression of c - fos, HSP70 and contributes to neuroprotective activities after cerebral ischemia.

关 键 词：脑缺血 热休克蛋白质70 基因 c—fos 二裂银杏 

分 类 号：R363[医药卫生—病理学]