人参二醇组皂苷对内毒素休克大鼠脑皮质TLR4 MRNA表达的影响  

Effects of PDS on TLR4 mRNA expression in cortex in endotoxic shock rats

作　　者：王健春[1] 孙晓霞[1] 王志[2] 袁兆新[3] 刘潇[2] 孙连坤[2] 李扬[2] 赵雪俭[2] 

机构地区：[1]吉林大学基础医学院机能科学实验中心,吉林长春130021 [2]吉林大学基础医学院病理生理学教研室,吉林长春130021 [3]长春医学高等专科学校,吉林长春130031

出　　处：《中国病理生理杂志》2005年第12期2431-2433,共3页Chinese Journal of Pathophysiology

基　　金：吉林省自然科学基金资助项目(NO.20030501)

摘　　要：目的:通过观察内毒素休克大鼠脑皮质中NOS活性、NO含量和TLR4 MRNA的表达及人参二醇组皂苷(PDS)对其的影响,探讨内毒素引起脑组织损伤的分子机制。方法:大鼠随机分为实验对照(CONTROL)组、内毒素休克(LPS)组、地塞米松(LPS+DEX)组和人参二醇组皂苷(LPS+PDS)组。大鼠静脉注射内毒素(4 MG/KG)4H后测定脑组织中NOS活性、NO含量及TLR4 MRNA的表达。结果:LPS+DEX组和LPS+PDS组NOS活性、NO2-/NO3-含量显著低于LPS组(P<0．05),TLR4 MRNA表达亦明显低于LPS组。结论:PDS能够下调脑组织中TLR4 MRNA的表达,降低 NOS活性、NO含量,对中枢神经系统具有保护作用。AIM： To explore the molecular mechanism of brain tissue injury induced by endotoxin. METHODS： Rats were divided into LPS, LPS ＋ DEX, LPS ＋ PDS and control group, respectively. NOS activity, NO content and TLR4 mRNA expression were assayed 4 h after intravenous injection of LPS. RESULTS： NOS activity, NO content and TLR4 mRNA expression in LPS ＋ DEX and LPS ＋ PDS groups were obviously lower than those in LPS group. CONCLUSION： PDS may provide protective effects on the central nervous system by down - regulating TLR4 expression, reducing NOS activity and NO content in the brain tissue.

关 键 词：内毒素类 人参 地塞米松 一氧化氮 一氧化氮合酶 大脑皮质 

分 类 号：R363[医药卫生—病理学]