足电击刺激对不同脑区及脑脊液IL-1Β含量的影响  

Effects of footshock on interleukin -1β content in different areas of the brain and cerebrospinal fluid in rats

作　　者：吕洋[1] 刘军[2] 任爱华[3] 赵华[1] 

机构地区：[1]吉林大学基础医学院生理教研室,吉林长春130021 [2]吉林大学第一临床医院消化内科,吉林长春130021 [3]北华大学医学院基础医学部生理教研室,吉林132013

出　　处：《中国病理生理杂志》2005年第12期2434-2437,共4页Chinese Journal of Pathophysiology

基　　金：吉林省科技厅资助课题(NO.990566)

摘　　要：目的:检测足电击对大鼠脑脊液及不同脑区白细胞介素-1Β(IL-1Β)含量的影响。方法:从第四脑室抽取脑脊液及分离小脑、延髓、海马、杏仁核、室旁核后,用酶联免疫吸附(ELISA)方法检测在足电击刺激下IL-1Β含量的变化。结果:①5 MIN电击(14 V,50 HZ)大鼠后足引起其脑脊液IL-1Β含量在30 MIN内明显高于对照组(P< 0．05),且20MIN足电击大鼠则在30-60MIN内引起脑脊液IL-1Β含量明显高于对照组(P<0．05)。②分别对大鼠后足电击5 MIN、10 MIN、15 MIN及20 MIN后,除20 MIN足电击引起大鼠杏仁核、室旁核IL-1Β含量高于对照组外(P< 0．05),延髓、小脑、海马内的IL-1Β含量均无明显改变。结论:足电击大鼠可以增加脑脊液及室旁核和杏仁核IL-1Β的含量。AIM： To study the influences of footshock on the content of interleukin - 1β in different areas of the brain and cerebrospinal fluid in rats. METHODS： The concentration of interleukin- 1β in cerebrospinal fluid from fourth ventricle, cerebullum, medulla, hippocampus, amygdala and paraventricular nucleus, were measured following the footshock by ELISA. RESULTS： ① Five minutes after footshock （14 V, 50 Hz） to the rearfoot of rats, the content of interleukin - 1β in the cerebrospinal fluid within 30 minutes was significantly higher than that in control group （ P 〈 0.05）, and 20 - minute footshock, the content of interleukin - 1β in cerebrospinal fluid between 30 - 60 minutes was significantly higher than that in control group （ P 〈 0.05）. ② After 5 min, 10 rain, 15 min, and 20 min footshock to the rear - foot of rats respectively, the content of interleukin - 1β was increased only in amygdala and paraventricular nucleus and only under 20 min footshock compared with the control group （ P 〈 0.05）, no significant change of interleukin - 1β in cerebnllum, medulla and hippocampus at any other time points was observed. CONCLUSION： Our results suggest that footshock to the rats increases interleukin -1β content in amygdale, paraventricular nucleus, and cerebrospinal fluid.

关 键 词：白细胞介素1 足 电击伤 脑脊髓液 杏仁核 下丘脑室旁核 

分 类 号：R392.9[医药卫生—免疫学]