NK细胞在重度子痫前期发病中的作用  

Effects of NK cells on the etiology of severe pre-eclampsia

作　　者：张展[1] 贾莉婷[1] 侯磊[1] 王雪梅[1] 常彩红[1] 杨如镜[1] 张曦[1] 刘胜洪[2] 郑芳[2] 龚非力[2] 

机构地区：[1]郑州大学第三附属医院检验科,河南郑州450052 [2]华中科技大学同济医学院免疫学系,湖北武汉430030

出　　处：《中国病理生理杂志》2005年第12期2457-2461,共5页Chinese Journal of Pathophysiology

基　　金：河南省科技攻关资助项目(NO.524410061)

摘　　要：目的:探讨NK细胞在重度子痫前期发病中的作用及其机制。方法:采集重度子痫前期晚孕、正常晚孕孕妇外周血及其新生儿脐血,PERCOLL分离NK细胞,SAP法计数,MTT法检测其增殖活性,[51CR]释放法检测其杀伤活性,免疫组织化学法检测胎盘蜕膜组织NK细胞数量及HLA-G蛋白表达水平。结果:①重度子痫前期患者外周血、蜕膜组织及其新生儿脐血中NK细胞数量均显著高于正常晚孕组(P<0．05);②重度子痫前期患者外周血及其新生儿脐血NK细胞增殖和杀伤活性均高于正常晚孕组(P<0．05);③重度子痫前期患者胎盘蜕膜HLA-G蛋白表达水平显著低于正常晚孕组(P<0．05)。结论:重度子痫前期患者NK细胞功能异常增强,可能参与疾病发生。AIM： To detect the effects of NK cells on the etiology of severe pre - eclampsia. METHODS： The peripheral blood and umbilical blood from severe pre - eclamptic patients and normal late pregnant women were collected. NK cells were seperated in percoll and counted by SAP. The proliferating ability of NK cells was measured by MTT and killing power of NK cells was determined by [^51Cr] releasing test. The immunohistochemistry method was used to detect the quantity of NK cells and the expression of HLA - G in placenta of them. RESULTS ： （ 1 ） Compared with that in normal late pregnant women, the counts of the NK cells in peripheral blood umbilical blood and decidua of severe pre - eclamptic patients were significantly higher （all P 〈 0.05）. （2） Compared with that in normal late pregnant women, proliferating and killing ability of NK cells in peripheral blood and umbilical blood of severe pre - eclamptic patients were significantly higher. （3） The expression of HLA - G protein in placenta of severe pre - eclampsia group was lower than that in normal group .（ P 〈 0.05）. CONCLUSION： The quantity of NK cells in severe pre- eclamptic patients is increased with stronger biologic function. They may be related with the pathogenesis of severe pre eclampsia.

关 键 词：子痫 杀伤细胞 天然 HIA—G抗原 蜕膜 

分 类 号：R363[医药卫生—病理学]