全反式维甲酸对人子宫内膜腺癌细胞增殖抑制作用的研究  

Study on the proliferation inhibition effect of all trans retinoic acid on human endometrial adenocarcinoma cell line

作　　者：常淑芳[1] 雷玲[1] 刘维超[1] 熊正爱[1] 

机构地区：[1]重庆医科大学附属第二医院妇产科,400010

出　　处：《重庆医科大学学报》2005年第6期787-790,共4页Journal of Chongqing Medical University

基　　金：本课题为重庆市卫生局科研基金资助项目(03-2-063)。

摘　　要：目的:探讨全反式维甲酸(All trans retinoic acid,ATRA)对人子宫内膜腺癌细胞株(RL95-2)的增殖抑制作用。方法:用不同浓度ATRA处理体外培养的RL95-2,分别在处理后不同的时间点,以MTT法检测ATRA对RL95-2增殖活性的影响;用流式细胞术检测经ATRA处理后的RL95-2细胞周期的变化;免疫组化方法检测细胞周期素A(Cyclin A)的改变;扫描电镜和透射电镜观察ATRA处理后的细胞形态变化。结果:ATRA可抑制RL95-2的增殖,且这种作用呈时间及浓度依赖性。FCM分析发现RL95-2细胞增殖被ATRA阻滞于G0／G1期。电镜观察到用药后细胞恶性表型消失。免疫组化发现AT- RA处理后48h,Cyclin A蛋白表达降低。结论:ATRA对子宫内膜癌细胞系RL95-2的生长有抑制作用,且这种抑制作用与 Cyclin A蛋白表达水平降低有关。Objective： To evaluate the action of all trans retinoic acid on human endometrial adenocarcinoma cell line RL95 -2. Methods ： The human endometrial adenocarcinoma cells （ RL95 - 2 ） cultured in vitro were treated with various concentrations of all trans retinoic acid（ATRA）. At specific time points after treatment with ATRA, the effect of ATRA on the proliferation of RL95 -2 cells was determined by MTT method, and Cyclin A was detected by immunohistochemistry. The morphological change of RL95 - 2 cells was observed by scanning electron microscope and transmission electron microscope. Results ： A time - dependent and dose - dependent proliferation inhibition was demonstrated in RL95 -2, and ATRA induced a G0/G1 cell cycle arrest. The malignant morphological characteristic of RL95 - 2 cells disappeared after treatment by ATRA. ATRA could down - regulate the expression of Cyclin A. Conclusion ： The proliferation inhibition effect of ATRA on RL95 - 2 cells cultured in vitro appears to be due to the down - regulation of Cyclin A. The results provide evidence for the potential effect of ATRA in the prevention and treatment of human endometrial adenocarcinoma.

关 键 词：全反式维甲酸 子宫内膜腺癌 治疗 

分 类 号：R737.3[医药卫生—肿瘤]