宫颈癌前病变中ΔNP63与HPV16 E7表达的研究  

Study on Expression of ANP63 and HPV16 E7 in Cervical Intraepithelial Neoplasia

作　　者：贾英[1] 吴瑾[1] 唐良萏[1] 

机构地区：[1]重庆医科大学附属第一医院妇产科,重庆400016

出　　处：《重庆医科大学学报》2005年第6期846-848,共3页Journal of Chongqing Medical University

摘　　要：目的:研究宫颈上皮内瘤变中ANP63和HPV16 E7 mRNA的表达情况,寻找用于临床评估CIN病变进展趋势的生物学标志物。方法:收集临床71例薄层液基细胞标本,采用SP免疫组化法检测细胞中ANP63的蛋白表达,RT-PCR法检测残留标本中HPV16 E7的mRNA转录情况。结果:1．ANP63定位于异常鳞状上皮细胞和腺细胞,在CIN中表达高于炎症组(P< 0．05);2．HPV16 E7在CIN中表达明显高于炎症组(P<0．05);3．CIN中ANP63与HPV16 E7的检测结果具有较好的一致性。结论:ANP63可作为CIN的生物学标志物辅助细胞学筛查;ANP63可间接反映HPV16 E7的转录情况,有利于临床评估高危型病毒HPV16的致瘤能力。objective： To examine the potential of ANP63 and HPV16 E7 as one of biomarkers for cervical premalignant lesions in ThinPrep smears, and to search for the identification of specific biomarkers for cervical intraepithelial neoplasia （CIN） as a new examining method to evaluate evolution of CIN. Method ： Streptavidin peroxidase （SP） method was used. Immunocytochemical analysis of △NP63 expression was performed in 71 ThinPrep smears. RT - PCR was used to detect the mRNA of HPV16 E7 in 71 ThinPrep liquid - based smears specimens. Result： Positive expression rate of △NP63 in CIN was higher than that in inflammation （P 〈 0.01 ）. The positive expression rate of HPV16 E7 in CIN was higher than that in inflammation （P 〈 0.01 ）. Conclusion： Overexpression of ANP63 can be a useful marker to reduce false - negative cytology interpretation. ANP63 should show indirectly the activation of HPV16 E7, which should be beneficial to evaluate the tumorigensis of HPV16.

关 键 词：CIN △NP63 HPV16 E7 

分 类 号：R737.3[医药卫生—肿瘤]