大鼠弥漫性脑损伤后Homer蛋白的表达及其意义  

Expression of Homer after diffuse brain injury in rats and its significance

作　　者：史铁钧[1] 费舟[1] 章翔[1] 黄卫东[1] 梁景文[1] 宋蕾[1] 

机构地区：[1]第四军医大学西京医院全军神经外科研究所,陕西西安710033

出　　处：《第四军医大学学报》2005年第24期2209-2211,共3页Journal of the Fourth Military Medical University

基　　金：国家自然科学基金(30270534)

摘　　要：目的研究大鼠弥漫性脑损伤后不同亚型Homer蛋白表达规律及其意义.方法SD大鼠105只,体质量(300±25)g,分为正常组、假手术组和弥漫性脑损伤(DBI)组,DBI组应用Marmarou大鼠致伤,假手术组不予以自由落体打击,其余处理同DBI组,分别在损伤后0.5,1,6,12,24,72,168h取大鼠主要脑区及核团裂解匀浆,进行各Homer蛋白的免疫印迹分析.结果DBI组神经组织中Homer1a蛋白的表达自伤后0.5h起持续至伤后168h,而正常及假手术组未见该蛋白阳性表达;Homer1b/c,Homer2a/b和Homer3在正常组、假手术组和DBI组均可见明确的阳性表达.结论大鼠弥漫性脑损伤后Homer1a蛋白的表达于损伤前后有显著变化,而Homer1b/c,Homer2a/b及Homer3于损伤前后无明显变化,提示Homer1a蛋白参与了神经元损伤过程,且可能对神经元损伤起到一定的保护作用.AIM： To study the expression and significance of Homer in diffuse brain injury（ DBI）of rat in vitro. METHODS： One hundred and five SD rats, weighing（300 ±25）g, were randomized to normal, sham-operated and DBI groups. The Marmarou rats were applied to DBI group and the sham-operated group was treated as DBI group except for “free fall” attack. The rats＇ main brain areas and nuclei were taken to detect for Homer by immunoblotting at 0.5, 1,6, 12, 24, 72 and 168 h after injury respectively. RESULTS： Homerla was positive in DBI group from 0.5 h to 168 h after injury but no Homerla was observed in normal group and sham-operated group. Homerlb/c, Homer2a/b and Homer3 were present in the normal, sham-operated and DBI groups. CONCLUSION： There is a significant change in Homerla expression before and after injury, but there is no significant change in Homerlb/c, Homer2a/b and Homer3 levels, which indicates that Homerla is in- volved in the neuronal injury and may protect neurons from insults.

关 键 词：HOMER 免疫印迹法 弥漫性脑损伤 大鼠 

分 类 号：R742.5[医药卫生—神经病学与精神病学]