N-乙酰基-丝氨酰-天门冬酰-赖氨酰-脯氨酸对血小板洐生生长因子介导的NIH3T3细胞增殖的抑制调节作用  

Inhibition of N-acetyl-seryl-aspartyl-lysyl-proline on NIH3T3 cells proliferation mediated by platelet-derived growth factor in vitro

作　　者：杨方[1] 赵丹[1] 马文东[1] 罗玲[1] 户万秘[1] 王丽萍[1] 朱曦玲[1] 孙树勋[1] 

机构地区：[1]华北煤炭医学院实验研究中心,唐山063000

出　　处：《解剖学杂志》2005年第6期618-620,681,共4页Chinese Journal of Anatomy

基　　金：河北省科技厅博士基金(04547002D-5)河北省教育厅基金(2003112)唐山市新医药基础研究重点实验室项目(04362001B)

摘　　要：目的:探讨N-乙酰基-丝氨酰-天门冬酰-赖氨酰-脯氨酸(AcSDKP)对血小板洐生生长因子(PDGF)介导的NIH3T3细胞增殖的抑制调节作用。方法:采用MTT法和免疫组化法检测NIH3T3细胞增殖和增殖细胞核抗原(PCNA)的表达。结果:在 10-10～10-8mol／L浓度范围内,AcSDKP对PDGF介导的NIH3T3细胞增殖均有抑制作用,并在10-9 mol／L浓度时其抑制作用最佳。PDGF对NIH3T3细胞PCNA的表达具有增强作用,而AcSDKP对PDGF介导的NIH3T3细胞PCNA的表达有显著抑制作用。结论:AcSDKP对PDGF介导的NIH3T3细胞增殖有明显抑制作用。Objective： To investigate whether N acetyl seryl-aspartyl lysyl proline （AcSDKP） can inhibit NIH3T3 cell proliferation mediated by platelet derived growth factor （PDGF）. Methods： Proliferation of NIH3T3 cells and expression of proliferating cells nuclear antigen （PCNA） were measured hy MTT assay and immunohistochemistry. Results： AcSDKP at the concentration of 10^- 10-10^-8 mol/L inhibited NIH3T3 cells proliferation mediated hy PDGF. 10^-3mol/L AcSDKP had the strongest inhibiting effect on NIH3T3 cells proliferation induced by PDGF. PDGF increased expression of PCNA in NIH3T3 cells. However, AcSDKP in hibited this effect mediated hy PDGF. Conclusion： AcSDKP can inhibit NIH3T3 cells proliferation mediated by PDGF.

关 键 词：N-乙酰基-丝氨酰-天门冬酰-赖氨酰-脯氨酸 血小板洐生生长因子 增殖细胞核抗原 NIH3T3细胞 细胞增殖 

分 类 号：R363[医药卫生—病理学]