FLI-1在小圆细胞肿瘤鉴别诊断中的应用  

Application of FLI-1 in differential diagnosis of small round cell tumor

作　　者：王宁[1] 郑晓刚[1] 石群立[1] 周晓军[1] 孟奎[1] 

机构地区：[1]南京军区南京总医院病理科,南京210002

出　　处：《临床与实验病理学杂志》2005年第6期646-649,共4页Chinese Journal of Clinical and Experimental Pathology

摘　　要：目的研究FLI-1和CD99在小圆细胞肿瘤中的表达情况,探讨二者在小圆细胞肿瘤鉴别诊断中的应用价值。方法对46例小圆细胞肿瘤进行FLI-1和CD99免疫组化标记,并结合临床与病理组织学进行对比研究。结果FLI-1在Ew ing肉瘤/外周原始神经外胚叶肿瘤(EW S/PNET)中阳性表达率为90.5%(19/21),在分化差的滑膜肉瘤、横纹肌肉瘤分别为14.3%(1/7)、22.2%(2/9),而在嗅神经母细胞瘤和间叶软骨肉瘤均无表达。CD99在EW S/PNET中阳性表达率为95.2%(20/21),在分化差的滑膜肉瘤、横纹肌肉瘤、嗅神经母细胞瘤和间叶软骨肉瘤分别为71.4%(5/7)、66.7%(6/9)、75.0%(3/4)和60%(3/5)。FLI-1标记在EW S/PNET的敏感性为90.5%,特异性为88%;而CD99在EW S/PNET的敏感性为95.0%,特异性为32%。FLI-1在EW S/PNET中的特异性明显高于CD99(P<0.05)。结论FLI-1在EW S/PNET诊断中的价值优于CD99,并且可用于小圆细胞肿瘤的鉴别诊断。Purpose To study the expression of FLI-1 and CD99 in small round cell tumor and to explore the utility of FLI-1 in differentiatial diagnosis of small round cell tumor. Methods 46 cases of small round cell tumor were immunohistochemically detected with FLI-1 and CD99 ,combined with clinical data and pathology. Results The positive expression rate of FLI-1 was 90. 5% （ 19/21 ） in Ewing＇ s sarcoma/peripheal primitive neuroectodermal tumor （ EWS/PNET）, 14. 3% （ 1/7 ） in poorly differentiated synovial sarcomas, 22. 2% （2/9） in rhabdomyosarcomas, 0% （0/5） in esthesioneuroblastomas and 0% （0/4） in mesenchymal chondrosarcomas, respectively. But CD99 expression was 95.0% （ 20/21 ） in EWS/PNET, 71.4% （ 5/7 ） in poorly differentiated synovial sarcomas, 66.7% （8/9） in rhabdomyosarcomas, 75.0% （3/4） in esthesioneuroblastomas and 60% （ 3/5 ） in mesenchymal chondrosarcomas, respectively. The sensitivity and specificity of FLI-1 in EWS/PNET were 90. 5% and 88% , but those of CD99 in EWS/PNET were 95.0% and 32% , respectively. The specificity of FLI-1 in EWS/PNET was higher than that of CD99 （P 〈 0. 05 ）. Conclusions Immunohistochemical detection of FLI-1 is valuable in the diagnosis of EWS/PNET , and can be reliably applied to the differential diagnosis of small round cell tumors.

关 键 词：小圆细胞肿瘤 肉瘤 Ewing 神经外胚瘤 原始 诊断 鉴别 免疫组织化学 FLI-1 CD99 

分 类 号：R730.26[医药卫生—肿瘤]