木犀草素对实验性肺纤维化大鼠组织中TGF-β1mRNA表达的影响  

Effects of luteolin on expression of TGF-β1 mRNA in rats with pulmonary fibrosis

作　　者：龚国清[1] 钱之玉[1] 周曙[1] 

机构地区：[1]中国药科大学药理学教研室,江苏南京210009

出　　处：《中国药理学通报》2005年第12期1466-1469,共4页Chinese Pharmacological Bulletin

摘　　要：目的研究木犀草素对肺纤维化大鼠肺组织中转化生长因子β(TGFβ1)mRNA的表达的影响。方法大鼠气管内灌注博来霉素致大鼠肺纤维化病变,同时ig给予40mg·kg-1的木犀草素连续14d,观察肺重量指数和羟脯氨酸含量的变化;用RTPCR测定肺组织中TGFβ1mRNA表达水平。结果木犀草素可降低重量指数和羟脯氨酸含量,延缓肺纤维化进程,并抑制TGFβ1mRNA的表达。结论木犀草素抗肺纤维化作用与其抑制肺组织中TGFβ1mRNA表达有关。Aim To study the effects of luteolin on the expression of TGF-β1 mRNA in rats with pulmonary fibrosis, and elaborate the molecular mechanism of luteolin in pulmonary fibrosis therapy. Methods The model of pulmonary fibrosis was established through instilling bleomycin intratracheally. After luteolin treatment, the pulmonary index and the content of hydroxyproline （HYP） were observed. The levels of TGF-β mRNA in pulmonary tissues were determined with RT-PCR. Results The pulmonary index and the content of HYP decreased markedly in pulmonary fibrosis rats treated with luteolin. Furthermore, luteolin inhibited evidently the expression of TGF-β1 mRNA in lung tissues with pulmonary fibrosis. Conclusion The molecular mechanism of luteolin in pulmonary fibrosis therapy was associated with inhibition of the expression of TGF-β1 mRNA.

关 键 词：木犀草素 肺纤维化 转化生长因子-Β1 

分 类 号：R-332[医药卫生] R282.71