免疫刺激对大鼠海马CA2、3区nNOS和c-Fos表达的影响  

Effects of Immune Stimulation on the Expression of nNOS and c-Fos in Hippocampal CA2 and CA3 Regions of Rat

作　　者：韩秀引[1] 王蕾[2] 马玉琼[2] 欧可群[2] 陈文玉[2] 

机构地区：[1]复旦大学上海医学院人体解剖与组织胚胎学系,上海200032 [2]四川大学组织胚胎学与神经生物学教研室,四川成都610041

出　　处：《解剖科学进展》2005年第4期287-290,294,共5页Progress of Anatomical Sciences

基　　金：国家自然科学基金(39700075);国家教委博士学科点专项基金资助项目(1999063002)

摘　　要：目的通过观察细菌脂多糖(LPS)对海马CA2、3区nNOS和c-Fos表达的影响,探讨海马CA2、3区的免疫调节作用及其与NO和c-Fos蛋白的相关性。方法实验组SD大鼠腹腔注射LPS,对照组注射生理盐水,2.5 h后,采用RT-PCR方法检测大鼠海马CA2、3区nNOS mRNA、原位杂交技术检测该区c-Fos mR-NA,免疫组化方法分别检测该区nNOS和c-Fos的表达变化。结果对照组和腹腔注射LPS组大鼠海马CA2、3区的nNOS,c-Fos及其mRNA的OD值如下:nNOS mRNA(0.283±0.09,0.476±0.03),nNOS(0.653±0.97,1.155±0.12),c-Fos mRNA(1.031±0.99,1.326±0.91),c-Fos(0.426±0.16,0.830±0.14);LPS使大鼠海马CA2、3区nNOS和c-Fos mRNA及表达产物含量均上调。结论海马CA2、3区在机体的免疫应激反应中起重要调节作用,NO和c-Fos蛋白可能是该调节过程中的重要信使分子。Objective To observe the effects of lipopolysacharide （LPS） on the expression of nNOS and c- Fos in hippocamapl CA2 - 3 regions and investigate the roles of NO and c-Fos in the neuroimmunomodulation of hippocampal CA2 - 3 regions. Methods SD rats of experiment group were intraperitoneally injected by LPS, control rats were treated by normal saline. 2.5h after treatment, RT-PCR was used to detect the expression of nNOS mRNA,in situ hybridization to detect c-FosmRNA,and immunohistochemistry to detect nNOS and c-Fos. Results The optic density （OD） values of nNOS,c-Fos and their mRNAs in rat hippocampal CA2 - 3 regions were showed as follows： nNOS mRNA （ 0. 283 ± 0.09,0. 476 ± 0.03 ）, nNOS （ 0. 653 ± 0. 97,1. 155 ± 0. 12 ）, c-Fos mRNA （ 1.031 ± 0.99, 1. 326 ±0. 91 ） ,c-Fos（0. 426 ±0. 16,0. 830 ±0. 14） in control and LPS groups respectively. The present results indicate that LPS upregulates the expressions of nNOS,c-Fos and their mRNA in rat hippocampal CA2 and CA3 areas. Conclusion Rats hippocampal CA2 and CA3 regions play an important role in the immunomodulating process in which NO and c-Fos are closely involved.

关 键 词：菌脂多糖 海马CA2区 NNOS C-FOS 海马CA3区 免疫刺激 

分 类 号：R322.81[医药卫生—人体解剖和组织胚胎学]