~99Tc^m-HL91脑显像与脑胶质瘤恶性程度、葡萄糖转运蛋白1型表达的关系  

The relationship between 99~Tc^m-HL91 imaging of malignancy of brain gliomas and Glutl expression

作　　者：刘保平[1] 郭鹏德 孙秉奇[1] 谢新立[1] 程兵[1] 

机构地区：[1]郑州大学第一附属医院核医学科,450052 [2]清华大学第一附属医院核医学科

出　　处：《中华核医学杂志》2005年第6期350-352,i0003,共4页Chinese Journal of Nuclear Medicine

基　　金：2002年度河南省科技攻关项目(223034400)

摘　　要：目的探讨^(99)Tc^m-4,9-二氮-3,3,10,10-四甲基十二烷-2,11-二酮肟(HL91)判断脑胶质瘤恶性程度的价值及其与葡萄糖转运蛋白1型(Glutl)表达的关系。方法 24例脑胶质瘤患者,注射^(99)Tc^m-HL91 10 min、2及4 h 后进行头颅前后位、后前位和左右侧位静态显像。在平面图像上,利用感兴趣区技术,分别计算不同时相肿瘤与对侧正常脑组织的放射性比值(T/N)。所有患者1周内均行手术治疗,将切除标本做 HE 染色、免疫组织化学检查。结果 HE 染色证实,24例患者中高度恶性17例,低度恶性7例。所有高度恶性和2例低度恶性患者^(99)Tc^m-HL91显像阳性,其余5例低度恶性患者为阴性。高度恶性组和低度恶性组10 min 及2、4 h 的 T/N 比值分别为1.775±0.386、1.861±0.388、1.881±0.434和1.067±0.125、1.140±0.009、1.110±0.139,组内三时相间 T/N 比值差异无显著性(P>0.05);低度恶性和高度恶性肿瘤 T/N 比值间差异有显著性(P<0.001)。17例高度恶性和3例低度恶性患者 Glutl 表达阳性。且 Glutl 的表达在高度恶性胶质瘤患者[(41.91±20.53)%]明显高于低度恶性患者[(8.88±13.85)%]。胶质瘤对^(99)Tc^m-HL91的浓聚与 Glutl 的表达呈明显相关性(r=0.888～0.964,平均 r=0.919,P<0.001)。结论 ^(99)Tc^m-HL91在高度恶性脑胶质瘤中有较高浓聚而在低度恶性胶质瘤中浓聚较少,可用于鉴别脑胶质瘤恶性程度;^(99)Tc^m-HL91在胶质瘤中的浓聚与 Glutl 的表达呈明显相关性。Objective To evaluate the relationship between ^99Tc^m-4,9-diaza-3,3,10,10-tetramethyldodecan-2,11-dione dioxime （HL91） imaging of malignancy of brain gliomas and Glutl expression. Methods Planar brain SPECT imaging was performed in 24 brain gliomas patients at 10 min, 2 and 4 h after injection of ^99Tc^m-HL91. Regions of interest （ROls） were drawn, and the radioactivity ratios of lesionto-normal tissue （T/N） were calculated. All patients were operated within a week and the resected samples were stained with hematoxylin and eosin （ HE ） and immunohistochemistry. Results ^99Tc^m-HL91 was highly uptaked in all 17 high malignant gliomas with positive HE staining and also in 2 of 7 low grade gliomas. T/N ratio of the high malignant and the low grade gliomas at 10 min, 2 and 4 h were 1. 775 ± 0. 386, 1. 861 ± 0. 388, 1.881 ± 0. 434 and 1. 067 ± 0. 125, 1. 140 ± 0. 009, 1.110 ± 0. 139, respectively, showed no significant difference （P 〉 0.05 ）between them in each group. However, there was significant difference between the low grade and the high malignant gliomas groups（ P 〈 0.001 ）. Besides, Glutl was expressed in all the high malignant gliomas and 3 of the low grade gliomas. And it was significantly higher in former group E （41.91 ± 20.53 ）% ] than in latter group [ （8.88 ± 13.85 ）% ]. Uptake of ^99Tc^m-HL91 and expressin of Glutl in the high malignant gliomas were strongly correlated（ r = 0. 888 ～0. 964, mean r = 0. 919, P 〈 0.1301 ）. Conclusion ^99Tc^m-HL91 had a selective concentration in high malignancy brain gliomas and its uptake and expression of Glutl were highly correlated.

关 键 词：脑肿瘤 体层摄影术 发射型计算机 单光子 单糖转运蛋白质类 HL91 

分 类 号：R739.4[医药卫生—肿瘤]